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Histopathological effects following short-term coexposure of Cyprinus carpio to nanoparticles of TiO2 and CuO

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Abstract

The aim of this research was to investigate the coexposure of nanoparticles of titanium dioxide (TiO2) and copper oxide (CuO) on the alterations of the gill, intestine, kidney, and liver tissues of carps (Cyprinus carpio). In this study, carps (length 23 ± 1.5 cm; weight 13 ± 1.3 g) were divided into six groups of 15 each and exposed to 2.5 and 5.0 mg L−1 of CuO nanoparticles (NPs), 10.0 mg L−1 of TiO2 NPs, and 2.5 and 5.0 mg L−1 of CuO NPs + 10.0 mg L−1 of TiO2 NP mixture. Fish were sampled for histopathological studies after hematoxylin-eosin staining. Results indicated that the more kinds of histopathology anomalies observed with CuO NP and TiO2 NP mixture were broadly of the same type as CuO NPs and TiO2 NPs alone, but the severity or incidence of injuries of gill, intestine, liver, and kidney of carps in the mixture of CuO NPs + TiO2 NPs was higher than that of each NP alone. Moreover, behavioral changes in carps exposed to CuO NP and TiO2 NP mixture such as hyperactivity, loss of balance, and convulsions were higher than those to CuO NPs and TiO2 NPs alone. In conclusion, the presence of TiO2 NPs enhanced the effects of NPs of copper oxide in terms of histopathological changes in carps.

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Acknowledgments

The data provided in this study was taken from first author Ph.D. dissertation project with grant number 14/33858. Also, the research was funded by the Kurdistan University of Medical Sciences.

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Authors and Affiliations

  1. Student Research Committee, Kurdistan University of Medical Sciences, Sanandaj, Iran

    Borhan Mansouri

  2. Environmental Health Research Center, Kurdistan University of Medical Sciences, Sanandaj, Iran

    Borhan Mansouri, Afshin Maleki, Behroz Davari, Behzad Shahmoradi, Ebrahim Mohammadi & Siros Shahsavari

  3. Department of Entomology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran

    Behroz Davari

  4. Department of Fisheries, Faculty of Natural Resources, University of Kurdistan, Sanandaj, Iran

    Seyed Ali Johari

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  1. Borhan Mansouri
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  2. Afshin Maleki
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  3. Behroz Davari
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  4. Seyed Ali Johari
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  5. Behzad Shahmoradi
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  7. Siros Shahsavari
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Correspondence to Behroz Davari.

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In this study, experimental procedures conformed to the Ethics Committee of the Kurdistan University of Medical Sciences (MUK.REC.1393.198).

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Mansouri, B., Maleki, A., Davari, B. et al. Histopathological effects following short-term coexposure of Cyprinus carpio to nanoparticles of TiO2 and CuO. Environ Monit Assess 188, 575 (2016). https://doi.org/10.1007/s10661-016-5579-6

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