The effects of heterotransplantation on bone marrow structures were studied in experimental male mice. Suspension of the bone marrow from feline femur (1 ml bone marrow diluted in isotonic saline, 1:2) was injected into the caudal vein. Heterotransplantation led to an increase in the level of serotonin involved in cell differentiation. In 2 days, the counts of all cells except erythroid cells decreased. Immunohistochemical analysis of Ki-67 marker within 40 min after transplantation showed a significant increase of cell proliferative activity in comparison with that in intact mice. At later terms, the expression of Ki-67 in bone marrow cells decreased, while the expression of apoptosis regulator P-53 increased, which was associated with the presence of numerous dead cells.
Similar content being viewed by others
References
Vorob’eva OV. Neurotransmitters biogenic amines in the bone marrow structures at the allografting. Morfol. Ved. 2016;24(4):89-91. Russian.
Vorob’eva OV. Dynamics of the Morphofunctional State of Bone Marrow Cell-Differons as a Hematopoietic Organ. Zh. Anat. Gistopatol. 2017;6(2):26-29. Russian.
Karkishchenko NN, Gracheva SV. Guidelines for Laboratory Animals and Alternative Models in Biomedical Technologies. Moscow, 2010. Russian.
Lyubovtseva LA, Borisov AV. The effect of 5-HT on bone marrow cell proliferation. Morphology and Luminescent Histochemistry. Cheboksary, 1983. P. 124-126. Russian.
Lyubovtseva IA, Vorobeva OV, Lyubovtseva EV. Microanalysis of neurotransmitters in the introduction of foreign bone marrow. Morfol. Ved. 2015;(3):41-45. Russian.
Lyubovtseva EV, Lyubovtseva LA, Gur’yanova EA, Golubtsova NN, Moskovskii AV, Russov AV. The local regulation of organs by bioamine-containing cells. Morfologiya. 2009;136(4):91b. Russian.
Nefedova VV, Inzhevatkin EV, Nefedov VP. Role of S2 receptors in the stimulatory effect of 5-HT on hemopoietic bone marrow stem cells. Bull. Exp. Biol. Med. 2002;133(5):419-420.
Chesnokova NP, Morrison VV, Ponukalina EV, Zhevak TN, Afanas’eva GA, Polutova NV, Nevvazhai TA. Hematopoiesis and its regulation at various stages of differentiation of bone marrow hematopoietic cells. Saratov. Nauch.-Med. Zh. 2012;8(3):711-719. Russian.
Shur VY, Samotrueva MA, Mazhitova MV, Trizno NN, Fayziev RM, Petrenko LV, Shur YV. 5-HT: biological properties and prospects of clinical application. Fundament. Issled. 2014;(7-3):621-629. Russian.
Falck B, Hillarp NA, Thieme G, Torp A. Fluorescence of catechol amines and related compounds condensed with formaldehyde. J. Histochem. Cytochem. 1982;9(1-6):XI-XV.
Gerdes J. Ki-67 and other proliferation markers useful for immunohistological diagnostic and prognostic evaluations in human malignancies. Semin. Cancer Biol. 1990;1(3):199-206.
Author information
Authors and Affiliations
Corresponding author
Additional information
Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 166, No. 11, pp. 648-651, November, 2018
Rights and permissions
About this article
Cite this article
Vorob’yova, O.V., Lyubovtseva, L.A. Cellular Composition and Expression of Cell Proliferation and Apoptosis Markers in Bone Marrow Cells after Heterotransplantation. Bull Exp Biol Med 166, 695–698 (2019). https://doi.org/10.1007/s10517-019-04420-4
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10517-019-04420-4