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Comparative Analysis of the Formation of γH2AX Foci in Human Mesenchymal Stem Cells Exposed to 3H-Thymidine, Tritium Oxide, and X-Rays Irradiation

  • Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)
  • Published:
Bulletin of Experimental Biology and Medicine Aims and scope

We performed a comparative study of the formation of γН2АХ foci (a marker of DNA doublestrand breaks) in human bone marrow mesenchymal stem cells after 24-h incubation with 3Н-thimidin and tritium oxide with low specific activities (50-800 MBq/liter). The dependence of the number of γH2AX foci on specific activity of 3H-thymidine was described by a linear equation y=2.21+43.45x (R2=0.96), where y is the number of γH2AX foci per nucleus and x is specific activity in 1000 MBq/liter. For tritium oxide, the relationship was described by a linear equation y=2.52+6.70x (R2=0.97). Thus, the yield of DNA double-strand breaks after exposure to 3H-thymidine was 6.5-fold higher than after exposure to tritium oxide. Comparison of the effects of tritium oxide and X-ray radiation on the yield of DNA double-strand breaks showed that the relative biological efficiency of tritium oxide in a dose range of 3.78-60.26 mGy was 1.6-fold higher than that of X-ray radiation. Improvement of the methods of analysis of DNA double-strand breaks repair foci is highly promising in the context of creation of highly sensitive biodosimetry technologies for tritium compounds in humans.

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References

  1. Alloni D, Cutaia C, Mariotti L, Friedland W, Ottolenghi A. Modeling dose deposition and DNA damage due to low-energy β(-) emitters. Radiat. Res. 2014;182(3):322-330.

    Article  CAS  Google Scholar 

  2. Bannister L, Serran M, Bertrand L, Klokov D, Wyatt H, Blimkie M, Gueguen Y, Priest N, Jourdain JR, Sykes P. Environmentally relevant chronic low-dose tritium and gamma exposures do not increase somatic intrachromosomal recombination in pKZ1 mouse spleen. Radiat. Res. 2016;186(6):539-548.

    Article  CAS  Google Scholar 

  3. Flegal M, Blimkie M, Roch-Lefevre S, Gregoire E, Klokov D. The lack of cytotoxic effect and radioadaptive response in splenocytes of mice exposed to low level internal β-particle irradiation through tritiated drinking water in vivo. Int. J. Mol. Sci. 2013;14(12):23 791-23 800.

    Article  Google Scholar 

  4. Halazonetis TD, Gorgoulis VG, Bartek J. An oncogene-induced DNA damage model for cancer development. Science. 2008;319:1352-1355.

    Article  CAS  Google Scholar 

  5. Harrison JD, Khursheed A, Lambert BE. Uncertainties in dose coefficients for intakes of tritiated water and organically bound forms of tritium by members of the public. Radiat. Prot. Dosimetry. 2002;98(3):299-311.

    Article  CAS  Google Scholar 

  6. Kim SB, Baglan N, Davis PA. Current understanding of organically bound tritium (OBT) in the environment. J. Environ. Radioact. 2013;126):83-91.

    Article  CAS  Google Scholar 

  7. Korzeneva IB, Kostuyk SV, Ershova LS, Osipov AN, Zhuravleva VF, Pankratova GV, Porokhovnik LN, Veiko NN. Human circulating plasma DNA significantly decreases while lymphocyte DNA damage increases under chronic occupational exposure to low-dose gamma-neutron and tritium beta-radiation. Mutat. Res. 2015;779:1-15.

    Article  CAS  Google Scholar 

  8. Kotenko KV, Bushmanov AY, Ozerov IV, Guryev DV, Anchishkina NA, Smetanina NM, Arkhangelskaya EY, Vorobyeva NY, Osipov AN. Changes in the number of doublestrand DNA breaks in Chinese hamster V79 cells exposed to gamma-radiation with different dose rates. Int. J. Mol. Sci. 2013;14(7):13,719-13,726.

    Article  Google Scholar 

  9. Kozlowski R, Bouffler SD, Haines JW, Harrison JD, Cox R. In utero haemopoietic sensitivity to alpha, beta or X-irradiation in CBA/H mice. Int. J. Radiat. Biol. 2001;77(7):805-815.

    Article  CAS  Google Scholar 

  10. Little MP, Lambert BE. Systematic review of experimental studies on 2008;47(1):71-93.

    CAS  Google Scholar 

  11. Osipov AN, Grekhova A, Pustovalova M, Ozerov IV, Eremin P, Vorobyeva N, Lazareva N, Pulin A, Zhavoronkov A, Roumiantsev S, Klokov D, Eremin I. Activation of homologous recombination DNA repair in human skin fibroblasts continuously exposed to X-ray radiation. Oncotarget. 2015;6(29):26,876-26,885.

    Google Scholar 

  12. Osipov AN, Pustovalova M, Grekhova A, Eremin P, Vorobyova N, Pulin A, Zhavoronkov A, Roumiantsev S, Klokov DY, Eremin I. Low doses of X-rays induce prolonged and ATM independent persistence of gammaH2AX foci in human gingival mesenchymal stem cells. Oncotarget. 2015;6(29):27 275-27 287.

  13. Sharma A, Singh K, Almasan A. H2AX phosphorylation: a marker for DNA damage. Methods Mol. Biol. 2012;920:613-626.

  14. Sutherland BM, Bennett PV, Sidorkina O, Laval J. Clustered damages and total lesions induced in DNA by ionizing radiation: oxidized bases and strand breaks. Biochemistry. 2000;39(27):8026-8031.

    Article  CAS  Google Scholar 

  15. Tsvetkova A, Ozerov IV, Pustovalova M, Grekhova A, Eremin P, Vorobyeva N, Eremin I, Pulin A, Zorin V, Kopnin P, Leonov S, Zhavoronkov A, Klokov D, Osipov AN. gamma-H2AX, 53BP1 and Rad51 protein foci changes in mesenchymal stem cells during prolonged X-ray irradiation. Oncotarget. 2017;8(38):64,317-64,329.

    Article  Google Scholar 

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Authors and Affiliations

  1. A. I. Burnazyan State Research Center Federal Medical Biophysical Center, Federal Medical-Biological Agency, Moscow, Russia

    N. Yu. Vorob’eva, O. A. Kochetkov, E. I. Yashkina, A. A. Osipov, D. I. Kabanov, P. P. Surin, V. G. Barchukov & A. N. Osipov

  2. N. N. Semenov Institute of Chemical Physics, Russian Academy of Sciences, Moscow, Russia

    M. V. Pustovalova & T. M. Blokhina

  3. N. M. Emanuel Institute for Biochemical Physics, Russian Academy of Sciences, Moscow, Russia

    A. K. Grekhova

Authors
  1. N. Yu. Vorob’eva
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  2. O. A. Kochetkov
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  3. M. V. Pustovalova
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  4. A. K. Grekhova
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  5. T. M. Blokhina
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  6. E. I. Yashkina
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  7. A. A. Osipov
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  8. D. I. Kabanov
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  9. P. P. Surin
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  10. V. G. Barchukov
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  11. A. N. Osipov
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Corresponding author

Correspondence to A. N. Osipov.

Additional information

Translated from Kletochnye Tekhnologii v Biologii i Meditsine, No. 3, pp. 205-208, September, 2018

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Vorob’eva, N.Y., Kochetkov, O.A., Pustovalova, M.V. et al. Comparative Analysis of the Formation of γH2AX Foci in Human Mesenchymal Stem Cells Exposed to 3H-Thymidine, Tritium Oxide, and X-Rays Irradiation. Bull Exp Biol Med 166, 178–181 (2018). https://doi.org/10.1007/s10517-018-4309-1

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  • DOI: https://doi.org/10.1007/s10517-018-4309-1

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