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Physical exercise alters hepatic morphology of low-density lipoprotein receptor knockout ovariectomized mice

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Abstract

To explore the effects of physical exercise on the liver of animals in menopause, we analyzed the histomorphometric parameters of the hepatic tissue in ovariectomized and dyslipidemic female mice. The animals were distributed in six groups (n = 5): sedentary control (SC), sedentary ovariectomized control (SOC), trained ovariectomized control (TOC), sedentary LDL knockout (LDL-S), sedentary ovariectomized LDL knockout (LDL-SO), and trained ovariectomized LDL knockout (LDL-TO). At the end of the experiment, the liver and the visceral adipose tissue (VAT) of animals were removed for morphometric and stereological studies. In the LDL-S and LDL-SO animals, both sedentary, results showed reduction in the area (µm2) and major and minor diameters (µm) of hepatocytes and reduction in the portions of large hepatocytes, and increase in the percentage of Kupffer cells. The trained group showed a tendency of increase in the area and diameter and in the percentage of hepatocytes, as well significant reduction in the percentage of Kupffer cells and interstitial tissue. We suggested that training can prevent cell and tissue damage caused by the process of increase in hepatic fat, lipoperoxidation, and tissue inflammation in animals with privation of estrogen and dyslipidemia, apparently reflecting a better metabolic response of the hepatic tissue in organisms undergoing training.

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Authors and Affiliations

  1. Stricto Sensu Postgraduate Program in Physical Education and Aging, Universidade São Judas Tadeu, São Paulo, Brazil

    Franciele Mercúrio Rodrigues, Jenifer Inácio Adélio, Vanessa Oliveira Santana, Elisabeth De Marco Ornelas, Romeu Rodrigues de Souza & Laura Beatriz Mesiano Maifrino

  2. Department of Histology, Embryology and Molecular Biology, Universidade Federal de Goiás (UFG), Goiania, Brazil

    Clever Gomes Cardoso

  3. Clinical Analysis Laboratory, Faculdade de Medicina do ABC-FMABC2000, Lauro Gomes Avenue, Santo André, SP, CEP 09060-650, Brazil

    Glaucia Luciano da Veiga & Fernando Luiz Affonso Fonseca

  4. Pharmaceutical Sciences Department of Universidade Federal de São Paulo-Unifesp, Diadema, SP, Brazil

    Fernando Luiz Affonso Fonseca

Authors
  1. Franciele Mercúrio Rodrigues
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  2. Jenifer Inácio Adélio
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  3. Vanessa Oliveira Santana
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  4. Elisabeth De Marco Ornelas
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  5. Romeu Rodrigues de Souza
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  6. Clever Gomes Cardoso
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  7. Glaucia Luciano da Veiga
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  8. Fernando Luiz Affonso Fonseca
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  9. Laura Beatriz Mesiano Maifrino
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Correspondence to Fernando Luiz Affonso Fonseca.

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Rodrigues, F.M., Adélio, J.I., Santana, V.O. et al. Physical exercise alters hepatic morphology of low-density lipoprotein receptor knockout ovariectomized mice. Med Mol Morphol 52, 15–22 (2019). https://doi.org/10.1007/s00795-018-0198-7

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  • DOI: https://doi.org/10.1007/s00795-018-0198-7

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