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In-vitro regulation of odontogenic gene expression in human embryonic tooth cells and SHED cells

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Abstract

The bud-to-cap stage transition during early tooth development is a time when the tooth-inducing potential becomes restricted to the mesenchyme. Several key genes, expressed in the mesenchyme at this stage, are an absolute requirement for the progression of tooth development. These include the transcription factors Msx1 and Pax9. The inductive potential of tooth mesenchyme cells is a key requisite for whole-tooth bioengineering and thus identification of cells that can retain this property following expansion in culture is an important as yet unresolved, goal. We show here that in-vitro culture of embryonic human tooth mesenchyme cells and SHED cells express low levels of PAX9 and MSX1 and that these levels can be significantly upregulated by activation of different signalling pathways. Such in-vitro manipulation may thus offer a simple way of maintaining/restoring/inducing the odontogenic-inducing capacity in mesenchymal cells.

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Acknowledgements

We are grateful to Dr. Dianne Gerelli of the HDBR for the human embryonic and fetal material that was provided by the Joint MRC (grant # G0700089)/ Wellcome Trust (grant # GR082557) Human Developmental Biology Resource (http://hdbr.org).

Financial support was provided by the NIHR Comprehensive Biomedical Research Centre.

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Authors and Affiliations

  1. Department of Craniofacial Development and Stem Cell Biology, Dental Institute, Kings College London, Floor 27, Tower Ring, Guy’s Hospital, SE1 9RT, London, UK

    Ana Angelova Volponi & Paul T. Sharpe

  2. BioNec, Section of Histology and Embryology, Faculty of Medicine, University of Palermo, Palermo, Italy

    Angelo Leone

  3. Department of Oral Surgery, Dental Institute, King’s College University of London, London, UK

    Tara Renton

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  1. Angelo Leone
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  2. Ana Angelova Volponi
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  3. Tara Renton
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  4. Paul T. Sharpe
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Correspondence to Paul T. Sharpe.

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Leone, A., Angelova Volponi, A., Renton, T. et al. In-vitro regulation of odontogenic gene expression in human embryonic tooth cells and SHED cells. Cell Tissue Res 348, 465–473 (2012). https://doi.org/10.1007/s00441-012-1379-7

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  • DOI: https://doi.org/10.1007/s00441-012-1379-7

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