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Prepro-tachykinin gene expression in the brain of the honeybee Apis mellifera

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Abstract

We have recently identified a tachykinin-related peptide (AmTRP) from the mushroom bodies (MBs) of the brain of the honeybee Apis mellifera L. by using direct matrix-assisted laser desorption/ionization with time-of-flight mass spectometry and have isolated its cDNA. Here, we have examined prepro-AmTRP gene expression in the honeybee brain by using in situ hybridization. The prepro-AmTRP gene is expressed predominantly in the MBs and in some neurons located in the optic and antennal lobes. cDNA microarray studies have revealed that AmTRP expression is enriched in the MBs compared with other brain regions. There is no difference in AmTRP-expressing cells among worker, queen, and drone brains, suggesting that the cell types that express the prepro-AmTRP gene do not change according to division of labor, sex, or caste. The unique expression pattern of the prepro-AmTRP gene suggests that AmTRPs function as neuromodulators in the MBs of the honeybee brain.

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Authors and Affiliations

  1. Department of Biological Sciences, Graduate School of Science, University of Tokyo, Bunkyo-ku, 113-0033, Tokyo, Japan

    Hideaki Takeuchi, Miyuki Sawata, Yuko Matsuo & Takeo Kubo

  2. Suntory Institute for Bioorganic Research, Wakayamadai, Mishima, 618-8503, Osaka, Japan

    Akikazu Yasuda, Yoshimi Yasuda-Kamatani & Terumi Nakajima

  3. DNA Chip Research, 134 Godo-cho, Hodogaya-ku, 240-0005, Yokohama, Japan

    Azusa Kato & Atsumi Tsujimoto

Authors
  1. Hideaki Takeuchi
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  2. Akikazu Yasuda
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  3. Yoshimi Yasuda-Kamatani
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  4. Miyuki Sawata
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  5. Yuko Matsuo
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  6. Azusa Kato
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  7. Atsumi Tsujimoto
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  8. Terumi Nakajima
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  9. Takeo Kubo
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Correspondence to Takeo Kubo.

Additional information

This work was supported by a Grant-in-Aid from the Bio-oriented Technology Research Advancement Institution (BRAIN)

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Takeuchi, H., Yasuda, A., Yasuda-Kamatani, Y. et al. Prepro-tachykinin gene expression in the brain of the honeybee Apis mellifera . Cell Tissue Res 316, 281–293 (2004). https://doi.org/10.1007/s00441-004-0865-y

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  • DOI: https://doi.org/10.1007/s00441-004-0865-y

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