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Imunomodulative effect of liposomized muramyltripeptide phosphatidylethanolamine (L-MTP-PE) on mice with alveolar echinococcosis and treated with albendazole

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Abstract

The effect of liposomized muramyltripeptide phosphatidylethanolamine (L-MTP-PE) administered separately or with anthelmintic albendazole (ABZ) on cellular immunity of mice with alveolar echinococcosis was studied. The proliferative activity of splenic T and B lymphocytes was the most stimulated after combined L-MTP-PE + ABZ therapy [from weeks 8 to 14 post-infection (p.i.)] that also induced a long-term development of protective Th1 response (the highest serum concentration of IFN-γ from weeks 8 to 18 p.i.). On the contrary, Th2 response (cytokine IL-5) in infected mice treated with L-MTP-PE was inhibited since week 8 p.i., but a significant long-term decrease in IL-5 concentration was found after combined L-MTP-PE + ABZ therapy until the end of the experiment (until week 26 p.i.). L-MTP-PE stimulated the production of superoxide anion (O2 −) by peritoneal macrophages from weeks 8 to 12 p.i., but the highest O2 − production was accordingly recorded after therapy L-MTP-PE + ABZ from weeks 8 to 18 p.i. Stimulation of cellular immunity of mice with alveolar echinococcis with L-MTP-PE and an interaction with ABZ’s anti-parasitic effect resulted in the greatest and long-term reduction of growth of Echinococcus multilocularis cysts in the host from week 10 p.i. until the end of the experiment.

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Acknowledgments

This work was supported by the Slovak Research and Development Agency under the contract No. APVV-51-027605, the Slovak-Italian Science and Technology Cooperation for 2004–2007 (project “08 TAL”) and by the Slovak VEGA agency, grant No. 2/7186/27. The experimental protocols complied with the current Slovak ethic law.

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  1. Parasitological Institute of the Slovak Academy of Sciences, Hlinkova 3, 040 01, Košice, Slovak Republic

    Emília Dvorožňáková, Jarmila Porubcová & Viliam Šnábel

  2. Pavol Jozef Šafárik University, Institute of Biology & Ecology, Faculty of Sciences, Moyzesova 11, 041 67, Košice, Slovak Republic

    Peter Fedoročko

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  1. Emília Dvorožňáková
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  2. Jarmila Porubcová
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  3. Viliam Šnábel
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Correspondence to Emília Dvorožňáková.

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Dvorožňáková, E., Porubcová, J., Šnábel, V. et al. Imunomodulative effect of liposomized muramyltripeptide phosphatidylethanolamine (L-MTP-PE) on mice with alveolar echinococcosis and treated with albendazole. Parasitol Res 103, 919–929 (2008). https://doi.org/10.1007/s00436-008-1077-2

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