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Adsorptive loss of secreted recombinant proteins in transgenic rice cell suspension cultures

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Abstract

Adsorptive loss of human cytotoxic T-lymphocyte antigen 4-immunoglobulin (hCTLA4Ig) in transgenic rice cell suspension cultures was investigated using glass flasks, plastic flasks, disposable vessels, and stainless steel vessels. When hCTLA4Ig was added to the glass flasks containing sterile AA medium, a rapid decrease in the concentration of hCTLA4Ig, independent on pH, was observed resulting in more than 90% of the protein loss within 1 h due to the surface adsorption. When the same experiments were performed on four different types of culture equipments mentioned above, the lowest adsorption level was observed in the plastic flasks and the highest level was observed in the glass flasks. The use of the plastic flasks retarded the adsorptive loss of hCTLA4Ig at the early stage of the protein production. There was a significant increase in the production of hCTLA4Ig when the flasks were coated with bovine serum albumin. However, the spike test of purified hCTLA4Ig at two different concentrations of 15 and 100 mg L−1 in 500-mL spinner flasks confirmed that the amount of hCTLA4Ig adsorbed was dependent on the surface area of the flasks but not on the concentrations. In conclusion, although the protein adsorption affected the total amount of the protein yielded to some extent, it could be regarded as a minor factor in transgenic plant cell cultures with higher titer.

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Acknowledgments

This work was financially supported by INHA University Research Grant (INHA-42476).

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Authors and Affiliations

  1. Department of Biological Engineering, Inha University, Incheon, 402-751, Korea

    Jun-Young Kwon, Kyoung-Hoon Lee, Su-Hwan Cheon, Hyun-Nam Ryu & Dong-Il Kim

  2. College of Pharmacy, The Ohio State University, Columbus, OH, 43210, USA

    Sun Jin Kim

Authors
  1. Jun-Young Kwon
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  2. Kyoung-Hoon Lee
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  3. Su-Hwan Cheon
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  4. Hyun-Nam Ryu
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  5. Sun Jin Kim
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  6. Dong-Il Kim
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Corresponding author

Correspondence to Dong-Il Kim.

Additional information

Communicated by B. Li.

A contribution to the Special Issue: Plant Molecular Pharming in 2012 and Beyond.

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Kwon, JY., Lee, KH., Cheon, SH. et al. Adsorptive loss of secreted recombinant proteins in transgenic rice cell suspension cultures. Plant Cell Rep 31, 551–560 (2012). https://doi.org/10.1007/s00299-011-1184-x

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  • DOI: https://doi.org/10.1007/s00299-011-1184-x

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