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High accumulation of dehydrodiconiferyl alcohol-4-β-d-glucoside in free and immobilized Linum usitatissimum cell cultures

  • Physiology and Biochemistry
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Abstract

As flaxseed mainly accumulates lignans (secoisolariciresinol diglucoside and matairesinol), these compounds were barely or not detected in plant cell suspensions initiated from Linum usitatissimum. In contrast, these cell suspensions were shown to accumulate substantial amounts of a neolignan identified as dehydrodiconiferyl alcohol-4-β-d-glucoside (DCG) (up to 47.7 mg g−1 DW). The formation of this pharmacologically active compound was evaluated as a function of cell growth and in relation to phytohormone balance of the culture media. After establishment of efficient culture conditions, production of DCG was investigated in immobilized plant cell suspensions initiated from plantlet roots of L. usitatissimum. The results indicate that immobilization enhances the DCG production up to 60.0 mg g−1 DW but depresses the cell growth resulting in no improvement of the total DCG yield. Nevertheless, with immobilized cell suspensions, a release of DCG into the medium is observed allowing an easier recovery.

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Abbreviations

BAP:

benzylaminopurine

COSY:

correlation spectroscopy

DCG:

dehydrodiconiferyl alcohol-4-β-d-glucoside

DMSO:

dimethylsulfoxide

DW:

dry weight

ESI-MS:

electrospray ionization mass spectometry

EtOH:

ethanol

FDA:

fluorescein diacetate

FW:

fresh weight

HMBC:

heteronuclear multiple bond correlation

HPLC:

high performance liquid chromatography

HSQC:

heteronuclear single quantum correlation

LS medium:

Linsmaier and Skoog medium

NAA:

naphthaleneacetic acid

NMR:

nuclear magnetic resonance

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Acknowledgements

S. C. wishes to thank the Conseil Regional de Picardie for financing his doctoral grant. We also thank INRA (Institut National de la Recherche Agronomique) for providing flax seeds and whole plants.

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Authors and Affiliations

  1. Biologie des plantes et contrôle des insectes ravageurs, EA 3900, Groupe de Phytotechnologie, Faculté de Pharmacie, 1 rue des Louvels, 80037, Amiens Cedex 1, France

    Jacques Attoumbré, Stéphane Charlet, Sylvie Baltora-Rosset, Sophie Raynaud-Le Grandic, Françoise Gillet, Lamine Bensaddek, François Mesnard & Marc-André Fliniaux

  2. Laboratoire de Biologie des Ligneux et des Grandes Cultures, UPRES EA 1207, Antenne Scientifique Universitaire de Chartres, 21 rue de Loigny la Bataille, 28000, Chartres, France

    Christophe Hano

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  1. Jacques Attoumbré
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  2. Stéphane Charlet
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  3. Sylvie Baltora-Rosset
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  4. Christophe Hano
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  5. Sophie Raynaud-Le Grandic
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  7. Lamine Bensaddek
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  8. François Mesnard
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  9. Marc-André Fliniaux
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Correspondence to Sylvie Baltora-Rosset.

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Communicated by M. S. Petersen

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Attoumbré, J., Charlet, S., Baltora-Rosset, S. et al. High accumulation of dehydrodiconiferyl alcohol-4-β-d-glucoside in free and immobilized Linum usitatissimum cell cultures. Plant Cell Rep 25, 859–864 (2006). https://doi.org/10.1007/s00299-006-0137-2

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  • DOI: https://doi.org/10.1007/s00299-006-0137-2

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