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Essential Gene Clusters Identified in Stenotrophomonas MB339 for Multiple Metal/Antibiotic Resistance and Xenobiotic Degradation

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Abstract

Stenotrophomonas MB339, a bacterium, which could potentially utilize aromatic compounds and tolerate different heavy metals was isolated from industrial wastewater. Subsequent experiments revealed strains ability to resist antibiotics ofloxacin, streptomycin, rifampicillin, erythromycin, ampicillin, clindamycin, and toxicants including As2+, Hg2+, Cu2+, Ni2+, Pb2+. The shotgun sequencing strategy, genome assembly and annotation uncovered specific features, which make this strain MB339 effectively promising to cope with highly contaminated conditions. This report presents isolate’s assembled genome and its functional annotation identifying a set of protein coding genes (4711), tRNA (69 genes), and rRNA (9 genes). More than 2900 genes were assigned to various Clusters of Orthologous Groups (COGs) and 1114 genes attributed to 37 different Koyoto Encyclopedia of Genes and Genomes (KEGGs) pathways. Among these annotated genes, eighteen were for key enzymes taking part in xenobiotic degradation. Furthermore, 149 genes have been assigned to virulence, disease, and defense mechanisms responsible for multidrug and metal resistance including mercury, copper, and arsenic operons. These determinants comprised genes for membrane proteins, efflux pumps, and metal reductases, suggesting its potential applications in bioremediation.

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Acknowledgements

This work was financially supported by Higher Education Commission of Pakistan under international research support initiative program (IRSIP). The whole genome sequence was done at Romaciotti Centre for Genomics, University of New South Wales, Sydney, Australia.

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Authors and Affiliations

  1. Microbiology and Biotechnology Research Labarotory, Department of Environmental Science, Fatima Jinnah Women University, Rawalpindi, Pakistan

    Fozia Aslam & Azra Yasmin

  2. Centre for Marine Bio-innovation & School of Biological, Earth and Environmental Sciences, The University of New South Wales, Sydney, NSW, 2052, Australia

    Torsten Thomas

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  1. Fozia Aslam
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  2. Azra Yasmin
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Correspondence to Azra Yasmin.

Additional information

Nucleotide sequence accession number: This Whole genome project has been deposited into GenBank under the accession no. MSLW00000000. The version described in this paper is version MSLW01000000.

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Aslam, F., Yasmin, A. & Thomas, T. Essential Gene Clusters Identified in Stenotrophomonas MB339 for Multiple Metal/Antibiotic Resistance and Xenobiotic Degradation. Curr Microbiol 75, 1484–1492 (2018). https://doi.org/10.1007/s00284-018-1549-2

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  • DOI: https://doi.org/10.1007/s00284-018-1549-2

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