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Roseovarius ponticola sp. nov., a novel lipolytic bacterium of the family Rhodobacteraceae isolated from seawater

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Abstract

A lipolytic, Gram-negative, aerobic, non-motile and pleomorphic bacterial strain, designated W-SW3T, was isolated from seawater around Wando, an island of South Korea, and its taxonomic position was determined using a polyphasic approach. Its optimal growth occurred at 30 °C, pH 7.0–8.0 and 2.0–4.0% (w/v) NaCl. Neighbour-joining phylogenetic tree of 16S rRNA gene sequences showed that strain W-SW3T belonged to the genus Roseovarius of the family Rhodobacteraceae, by showing the highest 16S rRNA gene sequence similarity value (97.3%) to the type strain of Roseovarius nanhaiticus. Strain W-SW3T exhibited 16S rRNA gene sequence similarities of 93.2–95.6% to the type strains of the other Roseovarius species. Ubiquinone-10 was found as the predominant ubiquinone and C18:1ω7c and C16:0 were found as the major fatty acids. Phosphatidylcholine, phosphatidylglycerol, diphosphatidylglycerol, one unidentified lipid and one unidentified aminolipid were major polar lipids detected. The DNA G + C content of strain W-SW3T was 63.9 mol%. Mean DNA–DNA relatedness values of strain W-SW3T with the type strains of R. nanhaiticus and R. antarcticus were 18 and 9%, respectively. The phenotypic properties, together with the phylogenetic and genetic data, made it possible strain W-SW3T to be separated from other recognized species of the genus Roseovarius. On the basis of the data presented, strain W-SW3T represents a novel species of the genus Roseovarius, for which the name Roseovarius ponticola sp. nov. is proposed. The type strain is W-SW3T (= KCTC 62424T = KACC 19615T = NBRC 113186T).

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Acknowledgements

This work was supported by the project on survey of indigenous species of Korea of the National Institute of Biological Resources (NIBR) under the Ministry of Environment (MOE) and by a grant from the KRIBB Research Initiative Program of the Republic of Korea.

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Authors and Affiliations

  1. Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, South Korea

    Sooyeon Park, Ji-Min Park & Jung-Hoon Yoon

  2. Korean Collection for Type Cultires (KCTC), Korea Research Institute of Bioscience and Biotechnology (KRIBB), 181 Ipsin-gil, Jeongeup, South Korea

    Jung-Sook Lee

Authors
  1. Sooyeon Park
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  2. Ji-Min Park
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  3. Jung-Sook Lee
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  4. Jung-Hoon Yoon
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Corresponding author

Correspondence to Jung-Hoon Yoon.

Additional information

Communicated by Erko Stackebrandt.

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain W-SW3T is MG748727. DPD (digital protologue’ database) Taxon number: TA00429.

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Park, S., Park, JM., Lee, JS. et al. Roseovarius ponticola sp. nov., a novel lipolytic bacterium of the family Rhodobacteraceae isolated from seawater. Arch Microbiol 201, 215–222 (2019). https://doi.org/10.1007/s00203-018-1599-7

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  • DOI: https://doi.org/10.1007/s00203-018-1599-7

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