Abstract
A method for preparation of magnetosensitive nanocomposites on the basis of surface-modified magnetite carrying immobilized cisplatin and monoclonal antibody CD 95 (a medico-biological nanorobot model) was developed. Adsorption and covalent immobilization of monoclonal antibody CD 95 and human normal immunoglobulin on nanocomposites comprising magnetite coated with poly(acryl amide) and γ-aminopropylsiloxane was studied. Isotherms of covalent attachment of oxidized immunoglobulin via formation of Schiff bases and non-specific (physical) adsorption of the normal immunoglobulin were compared. Kinetics of release of the immunoglobulin to a model environment was studied. Interaction of the prepared models of nanorobots with the cell line MCF-7 was studied. It was shown that use of magnetically driven nanocomposites carrying the anti-tumour drug and the monoclonal antibody CD 95 causes a synergic cytotoxic effect which exceeds the influence of the control doses up to 50%.
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References
Roco MC, Williams RS, Alivisatos P (2002) Nanotechnology research directions. Vision for nanotechnology R&D in the next decade, Vol. 156. Kluwer Academic Publishers, Dordrecht
Gubin SP, Koksharov YuA, Khomutov GB, Yurkov GYu (2005) Magnetic nanoparticles: methods of preparation, structure, and properties. Adv Chem. 74(4):539–574 (in Russian)
Shpak AP, Gorbyk PP (2007) Physico-chemistry of nanomaterials and supramolecular structures. 1. Naukova Dumka, Kyiv (in Russian)
Turanskaya SP, Turov VV, Gorbyk PP (2007) Magnetic nanoparticles and nanocomposites in diagnostics and treatment of diseases. In: Chemistry, physics, and technology of surface: interagency research works collection. Institute of Surface Chemistry of the NAS of Ukraine, 13:272–294 Naukova Dumka, Kyiv (in Russian)
Levy L, Sahoo Y, Kim K-S, Bergey JE (2002) Synthesis and characterization of multifunctional nanoclinics for biological applications. Chem Mater 4:3715–3721
Moiseenko VM (2002) Abilities of monoclonal antibodies in treatment of malignant tumors. Pract Oncol 3(4):253–260 (in Russian)
Sviridov VV (1987) Chemical precipitation of metals from aqueous solutions. Vysshaya shkola, Minsk (in Russian)
Sviridov VV (1996) Inorganic synthesis. Vysshaya shkola, Minsk (in Russian)
Mikhailik OM, Povstugar VI, Mikhailova SS (1991) Surface structure of finely dispersed iron powders. 1. Formation of stabilizing coating. Colloid Surf 52:315–324
Yasuda H (1988) Polymerization in plasma. Mir, Moscow (in Russian)
Salianov FA (1997) Basics of low temperature plasma physics, plasma devices and technologies. Nauka, Moscow (in Russian)
Dziuba NP (1987) Method of titration in non-aqueous solvents in analysis of medical means. Chem Pharm Ind. 2:1–17 (in Russian)
Tarutina LI, Pozdniakova FO (1986) Spectral analysis of polymers and auxiliary compounds. Khimia, Leningrad (in Russian)
Dekhant I, Danz R, Kimmer V, Shmolke R (1976) Infrared spectroscopy of high molecular weight polymers. Khimia, Moscow (in Russian)
Krylov OV, Kiselev VF (1981) Adsorption and catalysis on transition metals and their oxides. Khimia, Moscow (in Russian)
Petranovska AL, Fedorenko OM, Gorbyk PP, Chuiko OO, Chekhun VF, Dubrovin IV, Semko LS, Storozhuk LP, Abramov MV, Revo SL (2005) Development and properties of magnetosensitive nanocomposites for targeted transport of medical means. Nanosyst Nanomater Nanotechnol. 3(3):817–823 (in Ukrainian)
Semko LS, Ogenko VM, Revo SL, Mishenko VN et al (2002) Electric and magnetic properties of composite materials in the polyethylene-nanocrystalline nickel system. Funct Mater 9(3):513–518
Chuiko AA (2001) Chemistry of silica surface. 1, 2. UkrINTEI, Kyiv (in Russian)
Petranovska AL, Fedorenko OM, Storozhuk LP, Gorbyk PP, Chuiko OO, Dziubenko LS, Oranska OI (2006) Modification of magnetite nanoparticles with γ-aminopropyltriethoxysilane from solution. Reports of NAS of Ukraine 1:157–162 (in Ukrainian)
Storozhuk LP (2007) Synthesis and properties of polyfunctional magnetosensitive nanocomposites. Ph.D. thesis. Kyiv (in Ukrainian)
Wilson BM, Nakane PK (1978) Recent developments in the periodate method of conjugating horseradish peroxidase (HRPO) to antibodies. Immunofluoresc Rel Staining Techn 215–244
Shmanai VV, Nikolaeva TA, Vinokurova LG, Litoshka AA (2001) Oriented antibody immobilization to polystyrene macrocarriers for immunoassay modified with hydrazide derivatives of poly(meth)acrylic acid. BMC Biotechnol 1(4):128–133
Korshak VV, Shtilman MI (1984) Polymers in processes of immobilization and modification of natural compounds. Nauka, Moscow (in Russian)
Doson R (1991) Biochemists’ handbook. Nauka, Moscow (in Russian)
Gorbyk PP, Petranovska AL, Storozhuk LP, Dubrovin IV, Semko LS, Chekhun VF (2006) Medico-biological nanocomposites on the basis of magnetite: synthesis, modification, functionalization of surface for in vitro applications. In: Surface chemistry, physics, and technology: Interagency collection of research works. Institute of surface chemistry of the NAS of Ukraine. Naukova Dumka, Kyiv, 11–12:374–397 (in Ukrainian)
Mosmann T (1983) Rapid colorimetry assay for cellular growth and survival: application to proliferation and cytotoxic assays. J Immunol Methods 65:55–63
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Gorbyk, P. et al. (2009). Chemical Construction of Polyfunctional Nanocomposites and Nanorobots for Medico-biological Applications. In: Shpak, A., Gorbyk, P. (eds) Nanomaterials and Supramolecular Structures. Springer, Dordrecht. https://doi.org/10.1007/978-90-481-2309-4_6
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DOI: https://doi.org/10.1007/978-90-481-2309-4_6
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