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Comparative Analysis of Metal Uptake Potential of Hyphal Fusion Progenies of AMF and Their Parents

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Book cover Mycorrhiza - Nutrient Uptake, Biocontrol, Ecorestoration

Abstract

The main objective of the present study was to compare 15 arbuscular mycorrhizal fungal isolates (8 parents representing 3 Glomus species under the same genera Glomaceae and their 7 stable hyphal fusion progenies) for their heavy metal uptake potential so that they can be recommended for soil reclamation, environmental protection, and enhanced agricultural production. In vitro studies were conducted with AMF cultures grown in jars with minimal media amended with or without coal ash containing heavy metals. The experimental design was completely randomized with three replicates per treatment. Uptake of ten metals namely Al, Cd, Co, Cr, Cu, Mn, Ni, Pb, Si and Zn was analyzed by atomic absorption spectrophotometer. Study results showed that heavy metal uptake by hyphal fusion progenies and their parental isolate differs considerably among themselves and also for different metals. Hyphal fusion progeny 14 and parental isolate P46 showed high tolerance for a wide range of metals.

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Acknowledgements

This work was carried out as a part of the Ph.D thesis of the first author at TERI University, New Delhi. We wish to acknowledge the technical support and guidance provided by TERI University, New Delhi. We thank TERI, New Delhi for providing the infrastructural facilities. We also acknowledge with thanks the financial support obtained from Department of Biotechnology, New Delhi. Thanks are also acknowledged to the technical support provided by Dr. Kamlesh Kumar Shukla, Mr. U.G. Reddy and Mr. H.S. Uppal of TERI, New Delhi.

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Authors and Affiliations

  1. Department of Biotechnology, Ministry of Science & Technology, New Delhi, India

    Rajalakshmi Muralidharan

  2. Noble Research Institute, LLC, 2510 Sam Noble Parkway, Ardmore, OK, 73401, USA

    Prasun Ray

  3. The Energy and Resources Institute, Darbari Seth Block, IHC Complex, Lodhi Road, New Delhi, 110003, India

    Alok Adholeya

Authors
  1. Rajalakshmi Muralidharan
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  2. Prasun Ray
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  3. Alok Adholeya
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Correspondence to Alok Adholeya .

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Editors and Affiliations

  1. Amity Institute of Microbial Technology, Amity University Uttar Pradesh, Noida, Uttar Pradesh, India

    Ajit Varma

  2. Amity Institute of Microbial Technology, Amity University Uttar Pradesh, Noida, Uttar Pradesh, India

    Ram Prasad

  3. Amity Institute of Microbial Technology, Amity University Uttar Pradesh, Noida, Uttar Pradesh, India

    Narendra Tuteja

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Muralidharan, R., Ray, P., Adholeya, A. (2017). Comparative Analysis of Metal Uptake Potential of Hyphal Fusion Progenies of AMF and Their Parents. In: Varma, A., Prasad, R., Tuteja, N. (eds) Mycorrhiza - Nutrient Uptake, Biocontrol, Ecorestoration. Springer, Cham. https://doi.org/10.1007/978-3-319-68867-1_13

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