Abstract
Wood samples from a second generation Populus cross were shown to have different lignin contents and S/G ratios (S: syringyl-like lignin structures; G: guaiacyl-like lignin structures). The lignin contents varied from 22.7% to 25.8% and the S/G ratio from 1.8 to 2.3. Selected samples spanning these ranges were hydrolyzed with dilute (1%) sulfuric acid to release fermentable sugars. The conditions were chosen for partial hydrolysis of the hemicellulosic fraction to maximize the expression of variation among samples. The results indicated that both lignin contents and S/G ratio significantly affected the yield of xylose. For example, the xylose yield of the 25.8% lignin and 2.3 S/G (high lignin, high S/G) sample produced 30% of the theoretical yield, whereas the xylose yield of the 22.7% lignin and 1.8 S/G (low lignin, low S/G) was 55% of the theoretical value. These results indicate that lignin content and composition among genetic variants within a single species can influence the hydrolyzability of the biomass.
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References
Aden, A., Ruth, M., Ibsen, K., et al. (2002), NREL/TP-510-32438, National Renewable Energy Laboratory (NREL), Golden, C. O.
Dinus, R. J., Payne, P., Sewell, M. M., Chiang, V. L., and Tuskan, G. A. (2000), Crit. Rev. Plant Sci. 20, 51–69.
Boudet, A. M., Goffner, D., Marque, C., Teulieres, C., and Grima-Pettenati, J. (1998), Ag. Biotech. News Inf. 10, 295–304.
Vinzant, T. B., Ehrman, C. I., Adney, W. S., Thomas, S. R., and Himmel, M. E. (1997), Appl. Biochem. Biotechnol. 62, 99–104.
Chiang, V. L. and Funaoka, M. (1990), Holzforschung 44, 309–313.
Hu, W.-J., Harding, S. A., Lung, J., et al. (1999), Nat. Biotechnol. 17, 808–812.
Jouanin, L., Goujon, T., de Nadai, V., et al. (2000), Plant Physiol. 123, 1363–1374.
LaPierre, C., Pollet, B., Petit-Conil, M., et al. (1999), Plant Physiol. 119, 153–164.
Pena, L. and Seguin, A. (2001), Trends Biotechnol. 19, 500–506.
Pilate, G., Guiney, E., Holt, K., et al. (2002), Nat. Biotechnol. 20, 607–612.
Wullschleger, S., Yin, T. M., DiFazio, et al. (2005), Can J. Forest Res. 35, 1779–1789.
Tuskan, G. A., West, D., Bradshaw, H. D., et al. (1999), Appl. Biochem. Biotech. 77–79, 1–11.
Jung, H. G. and Deetz, D. A. (1993), In: Cell Wall Lignification and Degradability, Jung, H. G. Buxton, D. R., Hatfield, R. D., and Ralph, J., eds., Madison, W. I., pp. 315–346.
Fontaine, A. S., Bout, S., Barriere, Y., and Vermerris, W. (2003), J. Agric. Food Chem. 51, 8080–8087.
Reddy, M. S. S., Chen, F., Shadle, G., Jackson, L., Aljoe, H., and Dixon, R. A., (2005), PNAS, (in print).
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Davison, B.H., Drescher, S.R., Tuskan, G.A., Davis, M.F., Nghiem, N.P. (2006). Variation of S/G Ratio and Lignin Content in a Populus Family Influences the Release of Xylose by Dilute Acid Hydrolysis. In: McMillan, J.D., Adney, W.S., Mielenz, J.R., Klasson, K.T. (eds) Twenty-Seventh Symposium on Biotechnology for Fuels and Chemicals. ABAB Symposium. Humana Press. https://doi.org/10.1007/978-1-59745-268-7_35
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DOI: https://doi.org/10.1007/978-1-59745-268-7_35
Publisher Name: Humana Press
Print ISBN: 978-1-58829-866-9
Online ISBN: 978-1-59745-268-7
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