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Triplex-Mediated Gene Modification

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Chromosomal Mutagenesis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 435))

Abstract

Gene targeting with DNA-binding molecules such as triplex-forming oligonucleotides or peptide nucleic acids can be utilized to direct mutagenesis or induce recombination site-specifically. In this chapter, several detailed protocols are described for the design and use of triplex-forming molecules to bind and mediate gene modification at specific chromosomal targets. Target site identification, binding molecule design, as well as various methods to test binding and assess gene modification are described.

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Author information

Authors and Affiliations

  1. Department of Genetics, Yale University School of Medicine, New Haven, CT

    Erica B. Schleifman

  2. Department of Genetics and Department of Therapeutic Radiology, Yale University School of Medicine, New Haven, CT

    Joanna Y. Chin & Peter M. Glazer

Authors
  1. Erica B. Schleifman
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  2. Joanna Y. Chin
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  3. Peter M. Glazer
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Editor information

Editors and Affiliations

  1. Sigma-Aldrich Biotechnology, Sigma-Aldrich Corporation, St. Louis, Missouri

    Gregory D. Davis

  2. SAFC Biosciences, Sigma-Aldrich Corporation, St. Louis, Missouri

    Kevin J. Kayser

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© 2008 Humana Press Inc., Totowa, NJ

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Schleifman, E.B., Chin, J.Y., Glazer, P.M. (2008). Triplex-Mediated Gene Modification. In: Davis, G.D., Kayser, K.J. (eds) Chromosomal Mutagenesis. Methods in Molecular Biology, vol 435. Humana Press. https://doi.org/10.1007/978-1-59745-232-8_13

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  • DOI: https://doi.org/10.1007/978-1-59745-232-8_13

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-899-7

  • Online ISBN: 978-1-59745-232-8

  • eBook Packages: Springer Protocols

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