Abstract
The lung parenchyma is comprised of many cells including the structurally important stromal fibroblasts. Fibroblasts function to produce extracellular matrix and are important in the maintenance of alveolar epithelial cells. To understand the role of fibroblasts both in homeostasis and disease, we isolate fibroblasts and grow them in culture. Two methods are presented here for the isolation and maintenance of mouse primary lung fibroblasts.
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Edelman, B.L., Redente, E.F. (2018). Isolation and Characterization of Mouse Fibroblasts. In: Alper, S., Janssen, W. (eds) Lung Innate Immunity and Inflammation. Methods in Molecular Biology, vol 1809. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8570-8_5
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DOI: https://doi.org/10.1007/978-1-4939-8570-8_5
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-8570-8
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