Abstract
While drug induced endocytosis in the erythrocyte has been well documented,1–3 the role of endocytosis in encapsulation using hypotonic dialysis has been less understood. Kruse et al.4 reported that the drug methotrexate may be entrapped by endocytosis when carrier erythrocytes are prepared by hypotonic dialysis. Using conditions that favored endocytosis during encapsulation by hypotonic dialysis, DeLoach and co-workers5 were unable to increase the encapsulation of radiolabeled markers thus demonstrating that endocytosis did not play a significant role in the encapsulation process. Additionally, in counting vesicles which appeared within cells on transmission electron micrograph (TEM) sections they were unable to document any increase in endocytosis under those same conditions. Carrier cells with endocytic vesicles did not exceed 25% and were totally absent in cells sampled at the dialyzed cell stage.
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© 1992 Springer Science+Business Media New York
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Droleskey, R.E., Andrews, K., Chiarantini, L., DeLoach, J.R. (1992). Use of Fluorescent Probes for Describing the Process of Encapsulation by Hypotonic Dialysis. In: Magnani, M., DeLoach, J.R. (eds) The Use of Resealed Erythrocytes as Carriers and Bioreactors. Advances in Experimental Medicine and Biology, vol 326. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-3030-5_9
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DOI: https://doi.org/10.1007/978-1-4615-3030-5_9
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