Abstract
L-Kynurenine 3-monooxygenase is an FAD-dependent mixed function oxidase located in the mitochondrial outer membrane (Okamoto and Hayaishi, 1969). The purification of the mammalian enzyme has been done only from rat liver mitochondria (Horn et al., 1971; Nishimoto et al., 1977; 1979) and never from other mammalian livers. In this paper we describe the method for the purification of this enzyme from pig liver mitochondrial outer membrane. It consists of (1) the extraction of the enzyme from the Chaps*-insoluble outer membrane material by the nonionic detergent, β-Octylglucoside, (2) ammonium sulfate fractionation, (3) DEAE-Sepharose CL-6B chromatography, and (4) affinity chromatography (mercurial-Sepharose).
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© 1996 Plenum Press, New York
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Uemura, T., Hirai, K. (1996). Purification of L-Kynurenine 3-Monooxygenase from Pig Liver Mitochondrial Outer Membrane. In: Filippini, G.A., Costa, C.V.L., Bertazzo, A. (eds) Recent Advances in Tryptophan Research. Advances in Experimental Medicine and Biology, vol 398. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-0381-7_82
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DOI: https://doi.org/10.1007/978-1-4613-0381-7_82
Publisher Name: Springer, Boston, MA
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