Abstract
At elevated light intensities (greater than ~200 μE/[m2·s]), the kinetic imbalance between the rate of photon excitation and thermally activated electron transport results in saturation of the rate of photosynthesis. Since maximum terrestrial solar radiation can reach 200 μE/(m2·s), a significant opportunity exists to improve photosynthetic efficiency at elevated light intensities by achieving a kinetic balance between photon excitation and electron transport, especially in designed large-scale photosynthetic reactors in which a low-cost and efficient biomass production system is desired. One such strategy is a reduction in chlorophyll (chl) antenna size in relation to the reaction center that it serves. In this article, we report recent progress in this area of research. Light-saturation studies for CO2 fixation were performed on an antenna-deficient mutant of Chlamydomonas (DS521) and the wild type (DES15) with 700 ppm of CO2 in air. The light-saturated rate for CO2 assimilation in the mutant DS521 was about two times higher (187 μmol/[h·mg of chl]) than that of the wild type, DES15 (95 μmol/[h·mg of chl]). Significantly, a partial linearization of the light-saturation curve was also observed. These results confirmed that DS521 has a smaller relative chl antenna size and demonstrated that reduction of relative antenna size can improve the overall efficiency of photon utilization at higher light intensities. The antenna-deficient mutant DS521 can provide significant resistance to photoinhibition, in addition to improvement in the overall efficiency of CO2 fixation at high light. The experimental data reported herein support the idea that reduction in chl antenna size could have significant implications for both fundamental understanding of photosynthesis and potential application to improve photosynthetic CO2 fixation efficiency.
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References
Greenbaum, E. and Lee, J. W. (1998), in BioHydrogen, Zaborsky, O. R., ed., Plenum Press, NY, pp. 235–241.
Clayon, R. K. (1977), in Chlorophyll-Proteins, Reaction Centers, and Photosynthetic Membranes, Brookhaven Symposia in Biology Number 28, Olson, J. M. and Hind, G., eds., Brookhaven National Laboratory Associated Universities, Upton, NY, pp. 1–15.
Herron, H. A. and Mauzerall, D. (1972), Plant Physiol. 50, 141–148.
Melis, A., Neidhardt, J., and Benemann, J. R. (1999), J. Appl. Phycol. 10, 515–525.
Galloway, R. and Mets, C. (1989), Biochim. Biophys. Acta 975, 66–71.
Imbault, P., Wittemer, C., Johanningmeier, U., Jacobs, J. D., and Howell, S. H. (1988), Gene 73, 397–407.
Owens, T. G., Webb, S. P., Mets, L., Alberte, R. S., and Fleming, G. R. (1989), Biophys. J. 56, 95–106.
Sueoka, N. (1960), Proc. Natl Acad. Sei. USA 46, 83–91.
Owens, T. G., Webb, S. P., Mets, L., Alberte, R. S., and Fleming, G. R. (1987), Proc. Nati Acad. Sci. USA 84, 1532–1536.
Werst, M., Jia, Y., Mets, L., and Fleming, G. R. (1992), Biophys. J. 61, 868–878.
Nakajima, Y. and Ueda, R. (1999), J. Appl. Phycol. 11, 195–201.
Krause, G. H. and Weis, E. (1991), Annu. Rev. Plant Physiol. Plant Mol. Biol. 42, 313–349.
Schmid, G., Price, J. M., and Gaffron, H. (1966), J. Microscopie 5, 205–212.
Miron, A. S., Gomez, A. C., Camacho, F. G., Grima, E. M., and Chisti, Y. (1999), J. Biotechnol. 70, 249–270.
Grima, E. M., Sevilla, J. F., Perez, J. S., and Camacho, F. G., (1996), J. Biotechnol. 45, 59–69.
Fernandez, F. A., Camacho, F. G., Perez, J. S., Sevilla, J. F., and Grima, E. M. (1998), Biotechnol. Bioeng. 58, 605–616.
Kok, B. (1953), Algal Culture: From Laboratory to Pilot Plant, Carnegie Institute, Washington, DC, pp. 63–75.
Janssen, M., Kuijpers, T. C., Veldhoen, B., Ternbach, M. B., Tramper, J., Mur, L. R., and Wijffels, R. H. (1999), J. Biotechnol. 70, 323–333.
Watanabe, Y., Delanoue, J., and Hall, D. O. (1995), Biotechnol. Bioeng. 47, 261–269.
Watanabe, Y. and Hall, D. O. (1996), Energ. Convers. Manage. 37, 1321–1326.
Zhang, K., Kurano, N., and Miyachi, S. (1999), Appl. Microbiol. Biotechnol. 52, 781–786.
Myers, J. (1957), Encyclopedia of Chemical Technology, pp. 649–680.
Rorrer, G. L. and Mullikin, R. K. (1999), Chem. Eng. Sci. 54, 3153–3162.
Lee, C. G. and Palsson, B. O. (1995), J. Ferment. Bioeng. 79, 257–263.
Ogbonna, J. C., Soejima, T., and Tanaka, H. (1999), J. Biotechnol. 70, 289–297.
Fuentes, M. R., Sanchez, J. R., Sevilla, J. F., Fernandez, F A., Perez, J. S., and Grima, E. M. (1999), J. Biotechnol. 70, 271–288.
Hirata, S., Taya, M., Tone, S., and Hayashitani, M. (1997), Kagaku Kogaku Ronbun 23, 331–341.
Lee, Y. K., Ding, S. Y., Low, C. S., Chang, Y. C., and Chew, P. C. (1995), J. Appl. Phycol. 7, 47–51.
Hu, Q., Guterman, H., and Richmond, A. (1996), Biotechnol. Bioeng. 51, 51–60.
Ratchford, I. J. and Fallowfield, H. J. (1992), J. Appl. Phycol. 4, 1–9.
Borowitzka, M. A. (1999), J. Biotechnol. 70, 313–321.
Mann, C. C. (1999), Science 283, 310–314.
Mann, C. C. (1997), Science 277, 1038–1043.
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Lee, J.W., Mets, L., Greenbaum, E. (2002). Improvement of Photosynthetic CO2 Fixation at High Light Intensity Through Reduction of Chlorophyll Antenna Size. In: Finkelstein, M., McMillan, J.D., Davison, B.H. (eds) Biotechnology for Fuels and Chemicals. Applied Biochemistry and Biotechnology. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-4612-0119-9_3
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DOI: https://doi.org/10.1007/978-1-4612-0119-9_3
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