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  1. No Access


    Multiwell Plate Tools for Controlling Cellular Alignment with Grooved Topography

    In many tissues, cells must be aligned for proper function. This alignment can occur at the cellular and/or subcellular (protein/molecular) level. The alignment of cytoskeletal components, in fact, precedes wh...

    Camila Londono, John Soleas, Petra B. Lücker, Suthamathy Sathananthan (2014)

  2. No Access


    High-Throughput Cell Aggregate Culture for Stem Cell Chondrogenesis

    Cell aggregate culture is a widely used, reliable system for promoting chondrogenic differentiation of stem cells. A high-throughput cell pellet culture enables screening of various soluble factors for their e...

    Ahmad E. Abu-Hakmeh, Leo Q. Wan (2014)

  3. No Access


    Analyzing the Global Chromatin Structure of Keratinocytes by MNase-Seq

    Eukaryotic DNA is wrapped around histone octamers, known as nucleosomes, in an orderly fashion that provides the primary structure of chromatin organization. The compaction of DNA into nucleosomal repeats not ...

    Jason M. Rizzo, Satrajit Sinha Ph.D. (2014)

  4. No Access


    Multiscale Mathematical Modeling and Simulation of Cellular Dynamical Process

    Epidermal homeostasis is maintained by dynamic interactions among molecules and cells at different spatiotemporal scales. Mathematical modeling and simulation is expected to provide clear understanding and pre...

    Shinji Nakaoka (2014)

  5. No Access


    Genetic Modification in Human Pluripotent Stem Cells by Homologous Recombination and CRISPR/Cas9 System

    Genetic modification is an indispensable tool to study gene function in normal development and disease. The recent breakthrough of creating human induced pluripotent stem cells (iPSCs) by defined factors (Taka...

    Haipeng Xue, Jianbo Wu, Shenglan Li, Mahendra S. Rao, Ying Liu (2014)

  6. No Access


    Phase Variants of the Common Spatial Patterns Method

    Numerous studies on EEG data indicate that various brain processes are characterized by phase relationships between different regions of the brain. The development of analytic techniques that can provide a bet...

    Kenneth P. Camilleri, Owen Falzon, Tracey Camilleri, Simon G. Fabri (2014)

  7. No Access


    Accelerated Three-Dimensional Neuroepithelium Formation from Human Embryonic Stem Cells and Its Use for Quantitative Differentiation to Human Retinal Pigment Epithelium

    Successful applications of pluripotent stem cells to cell-based therapies will rely on rapid and efficient methods to differentiate cells toward the target cell type. While methods have been developed for the ...

    Yu Zhu, Sven Schreiter, Elly M. Tanaka (2014)

  8. No Access


    Graph-theoretic Indices of Evaluating Brain Network Synchronization: Application in an Alcoholism Paradigm

    Electroencephalographic signal synchronization studies have been a topic of increasing interest lately, using both linear and nonlinear formulations. In this direction a graph-theoretic approach devised to stu...

    Vangelis Sakkalis, Theofanis Oikonomou, Vassilis Tsiaras, Ioannis Tollis (2014)

  9. No Access


    Effective Brain Connectivity from Intracranial EEG Recordings: Identification of Epileptogenic Zone in Human Focal Epilepsies

    In the context of focal and drug-resistant epilepsy, surgical resection of the epileptogenic zone (EZ) may be the only therapeutic option for reducing or suppressing seizures. The aim of epilepsy surgery is th...

    Giulia Varotto, Laura Tassi, Fabio Rotondi, Roberto Spreafico, Silvana Franceschetti (2014)

  10. No Access


    Schizophrenia Assessment Using Single-Trial Analysis of Brain Activity

    In the quest for neurophysiological biomarkers that uniquely characterize schizophrenia subjects, auditory evoked potentials (EPs) have been extensively used during the past several decades. Typically, EPs are...

    George Zouridakis, Darshan Iyer, Javier Diaz (2014)

  11. No Access


    On the Effect of Volume Conduction on Graph Theoretic Measures of Brain Networks in Epilepsy

    It is well established that both volume conduction and the choice of recording reference (montage) affect the connectivity measures obtained from scalp EEG, in the time and frequency domains. A number of measu...

    Manolis Christodoulakis, Avgis Hadjipapas, Eleftherios S. Papathanasiou (2014)

  12. No Access


    Extracellular Matrix Mimetic Peptide Scaffolds for Neural Stem Cell Culture and Differentiation

    Self-assembled peptide nanofibers form three-dimensional networks that are quite similar to fibrous extracellular matrix (ECM) in their physical structure. By incorporating short peptide sequences derived from...

    Busra Mammadov, Mustafa O. Guler, Ayse B. Tekinay (2014)

  13. No Access


    Protocol for Serial Cultivation of Epithelial Cells Without Enzymes or Chemical Compounds

    Deriving keratinocytes from epidermis or oral mucosa is a critical first step in the construction of cell-based tissue engineering and regenerative medicine applications. It would be advantageous to develop a ...

    Dongxia Ye, Antonio Peramo (2014)

  14. No Access


    Differentiation of Human Induced Pluripotent Stem Cells into a Keratinocyte Lineage

    Direct reprogramming of somatic cells into induced pluripotent stem cells (iPSCs) provides an opportunity to develop novel personalized treatment options for numerous diseases and to advance current approaches...

    Igor Kogut, Dennis R. Roop, Ganna Bilousova (2014)

  15. No Access


    Preparation and Delivery of 4-Hydroxy-Tamoxifen for Clonal and Polyclonal Labeling of Cells of the Surface Ectoderm, Skin, and Hair Follicle

    To study the cell behavior during morphogenesis of mouse surface ectoderm, skin, and hair follicles, we (1–3) have developed a new method to temporally induce clones that is based on a tamoxifen-dependent Cre ...

    Christine Chevalier, Jean-François Nicolas, Anne-Cécile Petit (2014)

  16. No Access


    Lipid Rafts and Detergent-Resistant Membranes in Epithelial Keratinocytes

    Our understanding of the plasma membrane has markedly increased since Singer and Nicolson proposed the fluid mosaic model in 1972. While their revolutionary theory of the lipid bilayer remains largely valid, i...

    Kathleen P. McGuinn, Mỹ G. Mahoney Ph.D. (2014)

  17. No Access


    Multifunction Co-culture Model for Evaluating Cell–Cell Interactions

    Interactions within the same cell population (homotypic) and between different cell types (heterotypic) are essential for tissue development, repair, and homeostasis. To elucidate the underlying mechanisms of ...

    Danielle R. Bogdanowicz, Helen H. Lu (2014)

  18. No Access


    Directed Differentiation of Human Embryonic Stem Cells into Neural Progenitors

    A variety of protocols have been used to produce neural progenitors from human embryonic stem cells. We have focused on a monolayer culture approach that generates neural rosettes. To initiate differentiation,...

    Erin Banda Ph.D., Laura Grabel (2014)

  19. No Access


    Direct Conversion of Pluripotent Human Embryonic Stem Cells Under Defined Culture Conditions into Human Neuronal or Cardiomyocyte Cell Therapy Derivatives

    Developing novel strategies for well-controlled efficiently directing pluripotent human embryonic stem cells (hESCs) exclusively and uniformly towards clinically relevant cell types in a lineage-specific manne...

    Xuejun H. Parsons (2014)

  20. No Access


    Generation of Epithelial Cell Populations from Human Pluripotent Stem Cells Using a Small-Molecule Inhibitor of Src Family Kinases

    Human pluripotent stem cells (hPSCs), under the right conditions, can be engineered to generate populations of any somatic cell type. Knowledge of what mechanisms govern differentiation towards a particular li...

    Joshua A. Selekman, Xiaojun Lian, Sean P. Palecek (2014)

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