36,141 Result(s)

within Protocol

Relevance Newest First Oldest First
previous Page of 1,808
and
  1. No Access

    Protocol

    Tuning Differentiation Signals for Efficient Propagation and In Vitro Validation of Rat Embryonic Stem Cell Cultures

    The rat is one of the most commonly used laboratory animals in biomedical research and the recent isolation of genuine pluripotent rat embryonic stem (ES) cell lines has provided new opportunities for applying...

    Stephen Meek, Linda Sutherland, Tom Burdon (2014)

  2. No Access

    Protocol

    Preanalytic Considerations for Molecular Genomic Analyses of Tissue

    Progress in the era of molecular characterization of patients, diseases, and drugs, relies on access to clinical biospecimens characteristic of the general population and of patients with specific disease stat...

    Maureen Cronin (2014)

  3. No Access

    Protocol

    The Impact of Pre-analytic Variables on Tissue Quality from Clinical Samples Collected in a Routine Clinical Setting: Implications for Diagnostic Evaluation, Drug Discovery, and Translational Research

    The increasing application of biomarkers in both clinical practice and research has led to a greater emphasis on optimal sample preparation for such assays. However, in most institutions, current practices inv...

    David G. Hicks M.D. (2014)

  4. No Access

    Protocol

    Generation, Expansion, and Differentiation of Cardiovascular Progenitor Cells from Human Pluripotent Stem Cells

    Cardiovascular progenitor cells (CVPCs) derived from human embryonic stem cells and human induced pluripotent stem cells represent an invaluable potential source for the study of early embryonic cardiovascular...

    Nan Cao, He Liang, Huang-Tian Yang (2014)

  5. No Access

    Protocol

    Isolation of Central Nervous System (CNS) Infiltrating Cells

    Leukocyte infiltration of the central nervous system (CNS) occurs under certain pathogenic conditions and most often results in severe disorders. Therefore, the isolation and analysis of such infiltrating cell...

    Ilgiz A. Mufazalov, Ari Waisman (2014)

  6. No Access

    Protocol

    In Vivo Stem Cell Transplantation Using Reduced Cell Numbers

    Dental pulp stem cell (DPSC) characterization is essential for regeneration of a dentin/pulp like complex in vivo. This is especially important for identifying the potential of DPSCs to function as stem cells....

    Takeo W. Tsutsui D.D.S., Ph.D. (2014)

  7. No Access

    Protocol

    Experimental Autoimmune Encephalomyelitis in Marmosets

    Experimental autoimmune encephalomyelitis (EAE) in the common marmoset, a small-bodied Neotropical primate, is a well-known and validated animal model for multiple sclerosis (MS). This model can be used for ex...

    S. Anwar Jagessar Ph.D., Karin Dijkman, Jordon Dunham, Bert A. ‘t Hart, Yolanda S. Kap (2014)

  8. No Access

    Protocol

    Clinical Electroencephalography in the Diagnosis and Management of Epilepsy

    This chapter deals with the clinical electroencephalography (EEG) and its application on epilepsy. EEG, its origin, its technical aspects comprising also the electrode placement, and the related montages are a...

    Nicholas-Tiberio Economou, Andreas V. Alexopoulos M.D., M.P.H. (2014)

  9. No Access

    Protocol

    Isolation and Expansion of Mesenchymal Stromal/Stem Cells from Umbilical Cord Under Chemically Defined Conditions

    From a perspective of manufacturer, procurement of bone marrow aspirates for isolation of mesenchymal stromal/stem cells (MSC) is challenging. The MSC isolated from adult donors have lower proliferation capaci...

    Heba Badraiq, Liani Devito, Dusko Ilic (2014)

  10. No Access

    Protocol

    Neuropathological Techniques to Investigate CNS Pathology in Experimental Autoimmune Encephalomyelitis (EAE)

    Neuropathological techniques such as conventional and immunohistochemical staining of paraffin-embedded tissue sections are instrumental for identification and characterization of aberrations of organ architec...

    Karin Steinbach, Doron Merkler (2014)

  11. No Access

    Protocol

    GMP-Compliant Human Adipose Tissue-Derived Mesenchymal Stem Cells for Cellular Therapy

    Stem cells, which can be derived from different sources, demonstrate promising therapeutic evidences for cellular therapies. Among various types of stem cell, mesenchymal stem cells are one of the most common ...

    Hamid-Reza Aghayan, Parisa Goodarzi, Babak Arjmand (2014)

  12. No Access

    Protocol

    Characterizing Pluripotent Stem Cells Using the TaqMan® hPSC ScorecardTM Panel

    Rapid technological developments for the efficient generation of footprint-free induced pluripotent stem cells (iPSC) enabled the creation of patient-specific iPSC for downstream applications in drug discovery...

    Jeffrey Fergus, Rene Quintanilla, Uma Lakshmipathy (2014)

  13. No Access

    Protocol

    ELISPOT Techniques

    The enzyme-linked immunospot (ELISPOT) assay is a widely used method for enumerating antigen-specific cytokine-producing or antibody-secreting immune cells. It is one of the most effective immunological and di...

    Niannian Ji, Thomas G. Forsthuber (2014)

  14. No Access

    Protocol

    Scalable Expansion of Human-Induced Pluripotent Stem Cells in Xeno-Free Microcarriers

    The expansion of human-induced pluripotent stem cells (hiPSCs) is commonly performed using feeder layers of mouse embryonic fibroblasts or in feeder-free conditions in two-dimensional culture platforms, which ...

    Sara M. Badenes, Tiago G. Fernandes, Carlos A. V. Rodrigues, Maria Margarida Diogo (2014)

  15. No Access

    Protocol

    cGMP-Compliant Transportation Conditions for a Prompt Therapeutic Use of Marrow Mesenchymal Stromal/Stem Cells

    We recently described conditions for safe 18-h manufacturer-to-patient transportation of freshly harvested hBM-MSC expanded under cGMP protocols using human platelet lysate (hPL), that allowed prompt use as an...

    Elena Veronesi, Jorge Sans Burns, Alba Murgia, Olivia Candini, Valeria Rasini (2014)

  16. No Access

    Protocol

    Positional Gene Cloning in Experimental Populations

    Positional cloning is a technique that identifies a trait-associated gene based on its location in the genome and involves methods such as linkage analysis, association mapping, and bioinformatics. This approa...

    Maja Jagodic, Pernilla Stridh (2014)

  17. No Access

    Protocol

    Media Fill for Validation of a Good Manufacturing Practice-Compliant Cell Production Process

    According to the European Regulation EC 1394/2007, the clinical use of Advanced Therapy Medicinal Products, such as Human Bone Marrow Mesenchymal Stem Cells expanded for the regeneration of bone tissue or Chon...

    Marta Serra, Livia Roseti, Alessandra Bassi (2014)

  18. No Access

    Protocol

    GMP-Grade Human Fetal Liver-Derived Mesenchymal Stem Cells for Clinical Transplantation

    Stem cell therapy seems a promising avenue in regenerative medicine. Within various stem cells, mesenchymal stem cells have progressively used for cellular therapy. Because of the age-related decreasing in the...

    Bagher Larijani, Hamid-Reza Aghayan, Parisa Goodarzi, Babak Arjmand (2014)

  19. No Access

    Protocol

    Standard Operating Procedure for the Good Manufacturing Practice-Compliant Production of Human Bone Marrow Mesenchymal Stem Cells

    According to the European Regulation (EC 1394/2007), Mesenchymal Stem Cells expanded in culture for clinical use are considered as Advanced Therapy Medicinal Products. As a consequence, they must be produced i...

    Livia Roseti, Marta Serra, Alessandra Bassi (2014)

  20. No Access

    Protocol

    Peripheral Blood Mononuclear Cells: Isolation, Freezing, Thawing, and Culture

    The work with peripheral blood mononuclear cells (PBMCs), which comprise lymphocytes and monocytes, is indispensable in immunological diagnostics and research. The isolation of PBMCs takes advantage of differe...

    Christine Riedhammer, Dagmar Halbritter, Robert Weissert (2014)

previous Page of 1,808