31,468 Result(s)
-
Protocol
Routine Histology Techniques for the Developing and Adult Central Nervous System
The preparation of tissue for histological study is a multi-step process in which potential loss of quality and the introduction of artifacts can occur during each step. Knowledge of the process and the potent...
-
Protocol
Modeling Binge Eating in Nonhuman Primates
Human and nonhuman primates are opportunistic feeders and are prone to both obesity and binge eating when food is abundant. This chapter describes procedures for studying binge eating using a foraging model th...
-
Protocol
Quantitative Proteomic Analysis of N-linked Glycoproteins in Human Tear Fluid
Human tear fluid is a complex biological fluid that is actually the extracellular fluid of the surface of the eye and can be considered a dilute protein solution. The quality of the tears are critical for visi...
-
Protocol
Capillary Electrophoresis for Protein Profiling of the Dimorphic, Pathogenic Fungus, Penicillium marneffei
Penicillium marneffei is an endemic, dimorphic fungus that exhibits very significant morbidity among immune compromised persons living or having traveled in Southeast Asia. The dimorphic nature of
-
Protocol
Characterization of STAT Self-Association by Analytical Ultracentrifugation
Multiple experimental tools have demonstrated that cytokine-induced STAT activation entails the transition of dimer conformations rather than de novo dimerization. In this chapter, we describe the utilization ...
-
Protocol
Identify Intronic MicroRNA with Bioinformatics
MicroRNAs (miRNAs) are single-strand nonprotein coding RAN with 18 to 25-nucleotides long. With complementary sequence to target messenger RNA (mRNA), miRNA regulates mRNA degradation and protein translation. ...
-
Protocol
Tools for Protein Posttranslational Modifications Analysis: FAK, a Case Study
Recent advances in mass spectrometry have resulted in an exponential increase in annotation of posttranslational modifications (PTMs). Just in the Swiss-Prot Knowledgebase, there are 89,931 of a total of 27 ch...
-
Protocol
Methods for Sperm-Mediated Gene Transfer
The transgenic technologies represent potent biotechnological tools that allow the generation of genetically modified animals useful for basic research and for biomedical, veterinary, and agricultural applicat...
-
Protocol
Retrograde Tracing Technique for Neonatal Animals
Tract tracing is a fundamental technique in neuroanatomy for examining fiber connections in the nervous system. After the introduction of horseradish peroxidase 40 years ago, many tracing substances have been ...
-
Protocol
Phenotypic Analysis of Bioactive Cells for Application in Regenerative Medicine
The following chapter outlines methodologies to phenotypically characterize primary cells for the use in tissue-engineered and regenerative medicine applications. Methods covered include analyzing cells using ...
-
Protocol
Covisualization of Methylcytosine, Global DNA, and Protein Biomarkers for In Situ 3D DNA Methylation Phenotyping of Stem Cells
DNA methylation and histone modifications are key regulatory mechanisms in cellular differentiation, and are skewed in complex diseases. Therefore, analyzing the higher nuclear organization of methylated DNA i...
-
Protocol
Ca2+ Imaging as a Tool to Assess TRP Channel Function in Murine Distal Nephrons
Transient receptor potential (TRP) channels are expressed in almost every segment of renal nephron from the glomerulus to the inner medullary collecting duct. Serving as a route for Ca2+ entry from the intratubul...
-
Protocol
Co-immunoprecipitation Protocol to Investigate Cytokine Receptor-Associated Proteins, e.g., Janus Kinases or Other Associated Signaling Proteins
Jak binding to cytokine receptors has been shown to be a complex and tight interaction. When studying loss-of-function or gain-of-function mutants of the Jaks or cytokine receptors it is often necessary to kno...
-
Protocol
Atomic Force Microscopy Functional Imaging on Vascular Endothelial Cells
One of the challenging tasks in molecular cell biology is to identify and localize specific binding sites on biological samples with high spatial accuracy (in order of several nm). During the past 5 years, sim...
-
Protocol
Primary Culture and Live Imaging of Adult Neural Stem Cells and Their Progeny
Adult neural stem cells (NSC) generate neurons throughout life, but little is known about the sequence of events involved in the transition from NSC to neurons. Studying the intermediary steps involved in the ...
-
Protocol
Biomarkers of Cell Senescence Assessed by Imaging Cytometry
The characteristic features of senescent cells such as their “flattened” appearance, enlarged nuclei and low saturation density at the plateau phase of cell growth, can be conveniently measured by image-assist...
-
Protocol
A Non-targeted Approach for Extended Liquid Chromatography-Mass Spectrometry Profiling of Free and Esterified Jasmonates After Wounding
Upon wounding or herbivory, plants quickly react by activating various defense mechanisms. A major part of these defenses is thought to be regulated by the jasmonate pathway through the induction of jasmonic a...
-
Protocol
Label-Free Quantitative Shotgun Proteomics Using Normalized Spectral Abundance Factors
In this chapter we describe the workflow used in our laboratory for label-free quantitative shotgun proteomics based on spectral counting. The main tools used are a series of R modules known collectively as th...
-
Protocol
Correlative Light–Electron Microscopy as a Tool to Study In Vivo Dynamics and Ultrastructure of Intracellular Structures
Correlative light–electron microscopy (CLEM) is a very effective technique that combines live-cell imaging and immuno-electron microscopy for ultrastructural morphological characterization of dynamic intracell...
-
Protocol
Measurements of Cytosolic Ion Concentrations in Live Cells
Here, we describe a series of methods suitable for the measurement of cytosolic ion concentrations in living plant cells using ion selective dyes. We describe procedures for the use of SBFI for the measurement...
