31,747 Result(s)
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Protocol
Routine Histology Techniques for the Developing and Adult Central Nervous System
The preparation of tissue for histological study is a multi-step process in which potential loss of quality and the introduction of artifacts can occur during each step. Knowledge of the process and the potent...
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Modeling Binge Eating in Nonhuman Primates
Human and nonhuman primates are opportunistic feeders and are prone to both obesity and binge eating when food is abundant. This chapter describes procedures for studying binge eating using a foraging model th...
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Quantitative Proteomic Analysis of N-linked Glycoproteins in Human Tear Fluid
Human tear fluid is a complex biological fluid that is actually the extracellular fluid of the surface of the eye and can be considered a dilute protein solution. The quality of the tears are critical for visi...
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Capillary Electrophoresis for Protein Profiling of the Dimorphic, Pathogenic Fungus, Penicillium marneffei
Penicillium marneffei is an endemic, dimorphic fungus that exhibits very significant morbidity among immune compromised persons living or having traveled in Southeast Asia. The dimorphic nature of
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Characterization of STAT Self-Association by Analytical Ultracentrifugation
Multiple experimental tools have demonstrated that cytokine-induced STAT activation entails the transition of dimer conformations rather than de novo dimerization. In this chapter, we describe the utilization ...
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Identify Intronic MicroRNA with Bioinformatics
MicroRNAs (miRNAs) are single-strand nonprotein coding RAN with 18 to 25-nucleotides long. With complementary sequence to target messenger RNA (mRNA), miRNA regulates mRNA degradation and protein translation. ...
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Tools for Protein Posttranslational Modifications Analysis: FAK, a Case Study
Recent advances in mass spectrometry have resulted in an exponential increase in annotation of posttranslational modifications (PTMs). Just in the Swiss-Prot Knowledgebase, there are 89,931 of a total of 27 ch...
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Methods for Sperm-Mediated Gene Transfer
The transgenic technologies represent potent biotechnological tools that allow the generation of genetically modified animals useful for basic research and for biomedical, veterinary, and agricultural applicat...
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Retrograde Tracing Technique for Neonatal Animals
Tract tracing is a fundamental technique in neuroanatomy for examining fiber connections in the nervous system. After the introduction of horseradish peroxidase 40 years ago, many tracing substances have been ...
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Phenotypic Analysis of Bioactive Cells for Application in Regenerative Medicine
The following chapter outlines methodologies to phenotypically characterize primary cells for the use in tissue-engineered and regenerative medicine applications. Methods covered include analyzing cells using ...
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Covisualization of Methylcytosine, Global DNA, and Protein Biomarkers for In Situ 3D DNA Methylation Phenotyping of Stem Cells
DNA methylation and histone modifications are key regulatory mechanisms in cellular differentiation, and are skewed in complex diseases. Therefore, analyzing the higher nuclear organization of methylated DNA i...
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Single-Walled Carbon Nanotube-Mediated Small Interfering RNA Delivery for Gastrin-Releasing Peptide Receptor Silencing in Human Neuroblastoma
Small interfering RNA (siRNA) has the potential to influence gene expression with a high degree of target gene specificity. However, the clinical application of siRNA therapeutics has proven to be less promisi...
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Ca2+ Imaging as a Tool to Assess TRP Channel Function in Murine Distal Nephrons
Transient receptor potential (TRP) channels are expressed in almost every segment of renal nephron from the glomerulus to the inner medullary collecting duct. Serving as a route for Ca2+ entry from the intratubul...
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Co-immunoprecipitation Protocol to Investigate Cytokine Receptor-Associated Proteins, e.g., Janus Kinases or Other Associated Signaling Proteins
Jak binding to cytokine receptors has been shown to be a complex and tight interaction. When studying loss-of-function or gain-of-function mutants of the Jaks or cytokine receptors it is often necessary to kno...
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Atomic Force Microscopy Functional Imaging on Vascular Endothelial Cells
One of the challenging tasks in molecular cell biology is to identify and localize specific binding sites on biological samples with high spatial accuracy (in order of several nm). During the past 5 years, sim...
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Primary Culture and Live Imaging of Adult Neural Stem Cells and Their Progeny
Adult neural stem cells (NSC) generate neurons throughout life, but little is known about the sequence of events involved in the transition from NSC to neurons. Studying the intermediary steps involved in the ...
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Biomarkers of Cell Senescence Assessed by Imaging Cytometry
The characteristic features of senescent cells such as their “flattened” appearance, enlarged nuclei and low saturation density at the plateau phase of cell growth, can be conveniently measured by image-assist...
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A Non-targeted Approach for Extended Liquid Chromatography-Mass Spectrometry Profiling of Free and Esterified Jasmonates After Wounding
Upon wounding or herbivory, plants quickly react by activating various defense mechanisms. A major part of these defenses is thought to be regulated by the jasmonate pathway through the induction of jasmonic a...
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Designing a GWAS: Power, Sample Size, and Data Structure
In this chapter we describe a novel Bayesian approach to designing GWAS studies with the goal of ensuring robust detection of effects of genomic loci associated with trait variation.
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Label-Free Quantitative Shotgun Proteomics Using Normalized Spectral Abundance Factors
In this chapter we describe the workflow used in our laboratory for label-free quantitative shotgun proteomics based on spectral counting. The main tools used are a series of R modules known collectively as th...
