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  1. No Access


    Protocols for Investigating the Microbial Communities of Oil and Gas Reservoirs

    Recent studies have shown that microorganisms and microbial activity in oil reservoirs and the associated production systems are much more prominent than was originally thought. These findings, in conjunction ...

    Nicolas Tsesmetzis, Michael J. Maguire, Ian M. Head, Bart P. Lomans (2016)

  2. No Access


    Genetic, Genomic and Systemic Analyses for Pure Cultures

    Although considered to be only a minute fraction of global biodiversity, the handling of the culturable microbiota is still essential for exploring the interface of the bacterial world with lipids, hydrocarbon...

    Víctor de Lorenzo (2016)

  3. No Access


    Introduction to Petroleum, Hydrocarbon, and Lipid Analysis

    In nature, petroleum, hydrocarbons, and lipids typically occur as complex mixtures of structurally diverse compounds with different physical and chemical properties. Notably, the occurrence of hydrocarbons and...

    Heinz Wilkes (2016)

  4. No Access


    High Pressure Cultivation of Hydrocarbonoclastic Aerobic Bacteria

    Hydrocarbon-degrading microorganisms capable to use hydrocarbons as a sole source of carbon and energy are widely distributed in marine environments (Yakimov et al., Curr Opin Biotechnol 18:257–266, 2007) occu...

    Smedile Francesco, La Cono Violetta, Genovese Maria, Ruggeri Giovacchino, Denaro Renata (2016)

  5. No Access


    Protocol for Start-Up and Operation of CSTR Biogas Processes

    There is currently a lack of consensus on how biogas processes should be started and run in order to obtain stable, efficient operation. Agreement on start-up and operating parameters would increase the qualit...

    A. Schnürer, I. Bohn, J. Moestedt (2016)

  6. No Access


    Protocol for Performing Protein Stable Isotope Probing (Protein-SIP) Experiments

    Protein-based stable isotope probing (protein-SIP) was developed to link microbial-specific metabolic function to phylogenetic information. The SIP-concept is based on the application of stable isotope-labeled...

    Nico Jehmlich, Martin von Bergen (2016)

  7. No Access


    Microbial Biomass and Community Composition Analysis Using Phospholipid Fatty Acids

    Phospholipid fatty acid (PLFA) analysis is a method that has been used for over 35 years and can be useful to the microbial ecologist by quantitatively providing: (1) an estimate of viable microbial biomass by...

    Aaron D. Peacock, David C. White (2016)

  8. No Access


    Constructing and Analyzing Metabolic Flux Models of Microbial Communities

    Here we provide a broad overview of current research in modeling the growth and behavior of microbial communities, while focusing primarily on metabolic flux modeling techniques, including the reconstruction o...

    José P. Faria, Tahmineh Khazaei, Janaka N. Edirisinghe, Pamela Weisenhorn (2016)

  9. No Access


    A Developmental and Reproductive Toxicology Program for Chemical Registration

    The goal of the chapter is to outline the process of testing molecules for potential developmental and reproductive toxicity (DART). Here, the entire process of DART testing is discussed, from the regulatory u...

    Kamin J. Johnson Ph.D., Bethany R. Hannas, Sue Marty, Carol L. Zablotny (2016)

  10. No Access


    A Brief Review on the Ecological Network Analysis with Applications in the Emerging Medical Ecology

    Ecology studies the relationship between organisms and their environment, and a well-accepted paradigm of ecological science is to divide the subject into molecular ecology, autecology, population ecology, com...

    Zhanshan (Sam) Ma, Chengchen Zhang, Qingpeng Zhang, Jie Li, Lianwei Li, Linyi Qi (2016)

  11. No Access


    Systems and Synthetic Biology in Hydrocarbon Microbiology: Applications

    Contemporary Microbial Biotechnology is experiencing a rapid transition between being a mostly trial-and-error endeavour towards becoming a quantitative and predictable branch of contemporary research. A key i...

    Víctor de Lorenzo (2016)

  12. No Access


    Compositional Characterization of Acidic Petroleum Constituents Using Negative Ion Mode Electrospray Ionization Coupled with Fourier Transform-Ion Cyclotron Resonance Mass Spectrometry

    Direct infusion Fourier transform-ion cyclotron resonance mass spectrometry (FT-ICR MS) is an emerging tool in the characterization of complex mixtures of organic compounds such as petroleum fluids. Ultra-high...

    Stefanie Poetz, Heinz Wilkes (2016)

  13. No Access


    Analysis of Cell Cycle Status of Murine Hematopoietic Stem Cells

    Hematopoietic stem cells (HSC) act as paradigmatic tissue-specific adult stem cells. While they are quiescent in steady-state conditions, they enter the cell cycle and proliferate in stress conditions and duri...

    Krzysztof Szade, Karolina Bukowska-Strakova, Monika Zukowska, Alicja Jozkowicz (2016)

  14. No Access


    Growth Factor-Free Pre-vascularization of Cell Sheets for Tissue Engineering

    The therapeutic efficacy of tissue-engineered constructs is often compromised by inadequate inosculation and neo-vascularization. This problem is considered one of the biggest hurdles in the field and finding ...

    Marina Costa, Rogério P. Pirraco, Mariana T. Cerqueira, Rui L. Reis (2016)

  15. No Access


    Metabolism and Drug–Drug Interaction in Pregnant Mother/Placenta/Fetus

    The pregnant woman and the presence of the fetus pose many challenges for proper and effective drug administration. The variety of physiological changes that takes place during pregnancy coupled together with ...

    Ali S. Faqi, Karsten A. Holm (2016)

  16. No Access


    Pigment Production Analysis in Human Melanoma Cells

    The human epidermal melanocyte is a highly specialized pigmented cell that serves to protect the epidermis from ultraviolet (UV) damage through the production of melanin, or melanogenesis. Misregulation in mel...

    Amelia Soto Hopkin, Elyse K. Paterson, Rolando Ruiz, Anand K. Ganesan (2016)

  17. No Access


    Reporter Systems to Study Cancer Stem Cells

    Cancer stem cells have been identified in primary tumors, patient derived xenografts, and established cancer cell lines. The development of reporters has enabled investigators to rapidly enrich for these cells...

    Caner Saygin, Mohamed Samour, Anastasia Chumakova, Awad Jarrar, Justin D. Lathia (2016)

  18. No Access


    Erratum to: Protocols for High-Throughput Isolation and Cultivation

    Karsten Zengler (2016)

  19. No Access


    Cultivation of Anaerobic Microorganisms with Hydrocarbons as Growth Substrates

    Microorganisms utilizing hydrocarbons anaerobically as organic growth substrates are grown in defined anoxic media with sulfate, nitrate, or other electron acceptors. Cultivation of such microorganisms is tech...

    F. Widdel (2016)

  20. No Access



    The ability of microorganisms to transform pollutants is well documented. However, in many cases microbial communities with the desired capabilities may develop too slowly or may not be sustained. In these cas...

    Heidi L. Gough, Jeppe L. Nielsen (2016)

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