43,666 Result(s)

within Protocol

Relevance Newest First Oldest First
previous Page of 2,184
  1. No Access


    Layer-by-Layer Assembly of Glucose Oxidase on Carbon Nanotube Modified Electrodes

    The use of enzymatically modified electrodes for the detection of glucose or other non-electrochemically active analytes is becoming increasingly common. Direct heterogeneous electron transfer to glucose oxida...

    Alice H. Suroviec in Enzyme Stabilization and Immobilization (2017)

  2. No Access


    Analysis of Posttranscriptional Regulation of CCN Genes

    Cells generally control the concentration of mRNA by transcriptional and posttranscriptional regulation, so the separate contributions of synthesis and degradation (“decay”) cannot be discriminated by the quan...

    Seiji Kondo, Satoshi Kubota, Masaharu Takigawa D.D.S., Ph.D. in CCN Proteins (2017)

  3. No Access


    Array CGH

    Array comparative genome hybridization, array CGH or aCGH, enables dense interrogation of specific loci or the entire human genome, and even allele-specific characterization of single nucleotide polymorphisms ...

    Eftychia Dimitriadou, Joris R. Vermeesch in Fluorescence In Situ Hybridization (FISH) (2017)

  4. No Access


    Functional Mapping of Somatostatin Receptors in Brain: In Vivo Microdialysis Studies

    In vivo microdialysis is a method used in neuroscience that permits continuous monitoring and quantification of neurotransmitters, their release, reuptake, and metabolism, in discrete brain regions in the awak...

    Andreas Kastellakis, James Radke in In Vivo Neuropharmacology and Neurophysiol… (2017)

  5. No Access


    Lyophilization of Adjuvanted Vaccines: Methods for Formulation of a Thermostable Freeze-Dried Product

    Lyophilization of vaccines is advantageous for the distribution and storage of thermally labile products, particularly in regions where cold chain management is difficult. To date, current lyophilized vaccines...

    Michelle Y. Chan, Timothy S. Dutill, Ryan M. Kramer in Vaccine Adjuvants (2017)

  6. No Access


    Interatomic Characterization of Protein–Protein Interactions in Membrane-Associated Mega-complexes

    The majority of cellular activities in all living cells are performed by proteins. Biochemical pathways, signal transduction cascades, and membrane-associated complexes of energy generation rely on the fine-tu...

    José Manuel Borrero-de Acuña, Lothar Jänsch in Hydrocarbon and Lipid Microbiology Protoco… (2017)

  7. No Access


    Cell Biological Assays for Measuring Odontogenic Activities of CCN Proteins

    In postnatal dentin formation, odontoblast differentiation occurs in the pulp tissue regenerative process under pathological condition. Odontoblasts and newly differentiated odontoblast-like cells beneath the ...

    Koichiro Muromachi, Hiroshi Sugiya, Nobuyuki Tani-Ishii in CCN Proteins (2017)

  8. No Access


    The Diffusive Properties of Hydrogel Microcapsules for Cell Encapsulation

    Hydrogel microcapsules have been used for decades to encapsulate cells and treat diseases ranging from neurodegenerative disorders to more systemic applications like Type I Diabetes. This cell encapsulation mo...

    D. M. Lavin, B. E. Bintz, C. G. Thanos Ph.D. in Cell Microencapsulation (2017)

  9. No Access


    Covalent Immobilization of Enzymes on Eupergit® Supports: Effect of the Immobilization Protocol

    A selection of best combination of adequate immobilization support and efficient immobilization method is still a key requirement for successful application of immobilized enzymes on an industrial level. Eupergit

    Zorica D. Knežević-Jugović, Sanja Ž. Grbavčić in Enzyme Stabilization and Immobilization (2017)

  10. No Access


    Photocontrol of the Src Kinase in Mammalian Cells with a Photocaged Intein

    Recently developed methods that can photochemically control protein activities and functions in live cells have opened up new opportunities for studying signaling networks at the cellular and subcellular level...

    Wei Ren, Hui-Wang Ai in Split Inteins (2017)

  11. No Access


    Sequence-Based Prediction of RNA-Binding Residues in Proteins

    Identifying individual residues in the interfaces of protein–RNA complexes is important for understanding the molecular determinants of protein–RNA recognition and has many potential applications. Recent techn...

    Rasna R. Walia, Yasser EL-Manzalawy in Prediction of Protein Secondary Structure (2017)

  12. No Access


    Effects of HDACi on Immunological Functions

    Histone deacetylase inhibitors (HDACi) are used as therapeutics for several B cell-derived malignancies. Furthermore, they have been shown to modulate the response of the immune system, like the B cell functio...

    René Winkler, Christian Kosan in HDAC/HAT Function Assessment and Inhibitor Development (2017)

  13. No Access


    Establishment and Characterization of Long-Term Cultures Derived from Primary Acute Myeloid Leukemia Cells for HDAC Inhibitor Research

    Histone deacetylase (HDAC) inhibitors are promising drugs. These agents lead to growth inhibition, cell cycle arrest, premature senescence, and apoptosis of malignant cells. Aim of our studies was to determine...

    Annette Romanski, Gesine Bug in HDAC/HAT Function Assessment and Inhibitor Development (2017)

  14. No Access


    High-Throughput Analysis of IgG Fc Glycopeptides by LC-MS

    This chapter contains a nanoscale liquid chromatography–mass spectrometry method for the glycoform profiling of the conserved Fc N-glycosylation site of monoclonal and polyclonal immunoglobulin G (IgG). It descri...

    David Falck, Bas C. Jansen, Noortje de Haan in High-Throughput Glycomics and Glycoproteom… (2017)

  15. No Access


    Affinity Purification of Proteins in Tag-Free Form: Split Intein-Mediated Ultrarapid Purification (SIRP)

    Proteins purified using affinity-based chromatography often exploit a recombinant affinity tag. Existing methods for the removal of the extraneous tag, needed for many applications, suffer from poor efficiency...

    Dongli Guan, Zhilei Chen in Split Inteins (2017)

  16. No Access


    A Novel Method to Quantify RNA–Protein Interactions In Situ Using FMTRIP and Proximity Ligation

    RNA binding proteins (RBP) and small RNAs regulate the editing, localization, stabilization, translation, and degradation of ribonucleic acids (RNAs) through their interactions with specific cis-acting element...

    C. Zurla, J. Jung, E. L. Blanchard, P. J. Santangelo Ph.D. in Enhancer RNAs (2017)

  17. No Access


    Purification of Polyhistidine-Tagged Proteins

    His-tagging is the most widespread and versatile strategy used to purify recombinant proteins for biochemical and structural studies. Recombinant DNA methods are first used to engineer the addition of a short ...

    Sinéad T. Loughran, Ronan T. Bree, Dermot Walls in Protein Chromatography (2017)

  18. No Access


    Application of FISH to Previously GTG-Banded and/or Embedded Cytogenetic Slides

    GTG-banded slides are often considered to be worthless for further molecular cytogenetic analyses. In this chapter we describe a simple protocol on how to reactivate such GTG-banded slides for further use in m...

    Thomas Liehr, Monika Ziegler in Fluorescence In Situ Hybridization (FISH) (2017)

  19. No Access


    Characterization of Archived Formalin-Fixed/Paraffin-Embedded or Cryofixed Tissue, Including Nucleus Extraction

    Interphase cytogenetics using archival tissue samples is a straightforward approach to obtain “cytogenetic information” from nuclei of solid tissue samples. It is the major tool to investigate specific numeric...

    Thomas Liehr in Fluorescence In Situ Hybridization (FISH) (2017)

  20. No Access


    Where the Name “GOR” Originates: A Story

    The GOR is a computer program to predict secondary structures in proteins when the amino acid sequence is known using the theory of information. The program GOR was named much later after publication in 1978 a...

    Jean Garnier in Prediction of Protein Secondary Structure (2017)

previous Page of 2,184