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Encyclopedia of Lipidomics pp 1–2Cite as

Automation: Cross-Contamination

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Synonyms

Impurity; Adulterant; Pollution; Taint

Cross-contamination is defined as the unintentional transfer of material from one sample to any other sample during analysis. Prevention of cross-contamination during lipidomics is essential to any automated system design. Contamination can occur at any point from sample preparation through to extraction and mass spectrometric analysis. Sample collection is a first point that cross-contamination may occur, and specific protocols for prevention are dependent on sample type and handling tools. For example, care must be taken to clean any tools that come in contact with sample, such as tissue dissection tools. Homogenization of tissue samples with low-throughput techniques such as glass-on-glass homogenizers or mortar and pestle systems introduce a substantial contamination risk, and therefore many lipidomics platforms now utilize high-throughput systems such as bead homogenization (Abbott et al. 2013). Plates, vials, and grinding media are...

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References

  • Abbott SK, et al. An improved high-throughput lipid extraction method for the analysis of human brain lipids. Lipids. 2013;48:307–18.

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Authors and Affiliations

  1. Faculty of Science, Medicine and Health, School of Biology, University of Wollongong, Northfields Ave, 2500, Wollongong, NSW, Australia

    Simon H. J. Brown

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  1. Simon H. J. Brown
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Correspondence to Simon H. J. Brown .

Editor information

Editors and Affiliations

  1. Yong Loo Lin School of Medicine Centre for Life Sciences, Singapore, Singapore

    Markus R. Wenk

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© 2015 Springer Science+Business Media Dordrecht

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Brown, S.H.J. (2015). Automation: Cross-Contamination. In: Wenk, M. (eds) Encyclopedia of Lipidomics. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-7864-1_49-1

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  • DOI: https://doi.org/10.1007/978-94-007-7864-1_49-1

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  • Publisher Name: Springer, Dordrecht

  • Online ISBN: 978-94-007-7864-1

  • eBook Packages: Springer Reference Biomedicine and Life SciencesReference Module Biomedical and Life Sciences

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