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Identification of Mammalian O-Mannosylated Glycopeptides

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Glycoscience: Biology and Medicine

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Abstract

O-Mannosylation is a vital protein modification that has crucial functions in cell-cell as well as cell-extracellular matrix adhesion in mammals. Although O-mannosyl glycans are abundant in the mammalian brain, the characterization of proteins bearing these glycans has been challenging. In this chapter, a straightforward LC-MS-based method involving α-mannosidase treatment is described to conclusively identify O-mannosylated proteins isolated from mammalian tissues.

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Author information

Authors and Affiliations

  1. Centre for Organismal Studies, University of Heidelberg, Im Neuenheimer Feld 360, 69120, Heidelberg, Germany

    Patrick R. Winterhalter & Sabine Strahl

  2. Center for Molecular Biology, University of Heidelberg, Im Neuenheimer Feld 282, 69120, Heidelberg, Germany

    Patrick R. Winterhalter & Thomas Ruppert

Authors
  1. Patrick R. Winterhalter
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  2. Thomas Ruppert
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  3. Sabine Strahl
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Corresponding author

Correspondence to Sabine Strahl .

Editor information

Editors and Affiliations

  1. Systems Glycobiology Research Group, RIKEN-Max Planck Joint Research Center for Systems Chemical Biology, Wako, Saitama, Japan

    Naoyuki Taniguchi

  2. Tokyo Metropolitan Institute of Gerontology, Tokyo Metropolitan Geriatric Hospital and Institute of Gerontology, Tokyo, Japan

    Tamao Endo

  3. Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA

    Gerald W. Hart

  4. Department of Biomolecular Systems, Max-Planck-Institute of Colloids and Interfaces, Potsdam, Germany

    Peter H. Seeberger

  5. Academica Sinica, NanKang, T´ai-pei, Taiwan

    Chi-Huey Wong

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© 2015 Springer Japan

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Winterhalter, P.R., Ruppert, T., Strahl, S. (2015). Identification of Mammalian O-Mannosylated Glycopeptides. In: Taniguchi, N., Endo, T., Hart, G., Seeberger, P., Wong, CH. (eds) Glycoscience: Biology and Medicine. Springer, Tokyo. https://doi.org/10.1007/978-4-431-54841-6_66

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