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Handbook of Electroporation pp 1–13Cite as

Atomic Force Microscopy to Explore Electroporation Effects on Cells

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Abstract

Atomic force microscopy (AFM) is more and more used in life science. Its ability to provide images of living cells as well as mechanical or adhesion maps makes it a technology that cannot be ignored. In the context of electroporation (EP) which undoubtedly affects the cell membrane or wall, a technology able to probe the cell surface is more than interesting. This chapter describes the principle of the AFM technology and especially the latest multiparametric imaging modes developed recently. It then demonstrates that AFM can be used to probe cell’s morphology modifications induced by electric pulses. We then show that EP modifies cell’s nanomechanical properties and that the actin cytoskeleton plays a major role in this process. Finally we shed light on the effects of EP on bacteria as probed by AFM. In this latest example, it must be noticed that no mechanical modifications are induced, but the adhesion properties of the bacteria are dramatically reduced by pulsed electric fields (PEFs). Altogether the chapter shows the interest of applying AFM on cells exposed to EP, in order to get a better fundamental understanding of EP effect on cells or bacteria.

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Authors and Affiliations

  1. LAAS-CNRS, Université de Toulouse, CNRS, Toulouse, France

    Etienne Dague

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  1. Etienne Dague
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Correspondence to Etienne Dague .

Editor information

Editors and Affiliations

  1. Faculty of Electrical Enginer, University of Ljubljana, Ljubljana, Slovenia

    Damijan Miklavcic

Section Editor information

  1. Institut de Pharmacologie et de Biologie Structurale, IPBS/CNRS UMR 5089, IPBS - CNRS UMR 5089, 205, route de Narbonne, BP 64182, 31077 Toulouse cedex 04, Toulouse, France

    Marie-Pierre Rols

  2. Department of Mechanical Engineering, University of California, 6124 Etcheverry Hall, Berkeley, CA 94720, Berkeley, California, USA

    Boris Rubinsky

  3. Department of Animal Production and Food Science, Faculty of Veterinary, University of Zaragoza, Tecnología de los Alimentos, C/ Miguel Servet, 177, 50013, Zaragoza, Spain

    Javier Raso

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© 2016 Springer International Publishing AG

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Dague, E. (2016). Atomic Force Microscopy to Explore Electroporation Effects on Cells. In: Miklavcic, D. (eds) Handbook of Electroporation. Springer, Cham. https://doi.org/10.1007/978-3-319-26779-1_134-1

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  • DOI: https://doi.org/10.1007/978-3-319-26779-1_134-1

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  • Publisher Name: Springer, Cham

  • Online ISBN: 978-3-319-26779-1

  • eBook Packages: Springer Reference Biomedicine and Life SciencesReference Module Biomedical and Life Sciences

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