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Laser Speckle Imaging of Cerebral Blood Flow

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Abstract

Monitoring the spatio-temporal characteristics of cerebral blood flow (CBF) is crucial for studying the normal and pathophysiologic conditions of brain metabolism. By illuminating the cortex with laser light and imaging the resulting speckle pattern, relative CBF images with tens of microns spatial and millisecond temporal resolution can be obtained. In this chapter, a laser speckle imaging (LSI) method for monitoring dynamic, high-resolution CBF is introduced. To improve the spatial resolution of current LSI, a modified LSI method is proposed. To accelerate the speed of data processing, three LSI data processing frameworks based on graphics processing unit (GPU), digital signal processor (DSP), and field-programmable gate array (FPGA) are also presented. Applications for detecting the changes in local CBF induced by sensory stimulation and thermal stimulation, the influence of a chemical agent on CBF, and the influence of acute hyperglycemia following cortical spreading depression on CBF are given.

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Acknowledgments

This work was supported by Science Fund for Creative Research Group of China (Grant No.61121004), the National Natural Science Foundation of China (Grant Nos. 30970964, 30070215, 30170306, 60178028), NSFC for distinguished young scholars (Grant No. 60025514) and the Program for New Century Excellent Talents in University (Grant No. NCET-08-0213). It was also partly supported by RFBR grants No. 11-02-00560-а and No. 12-02-31204, grant № 224014 PHOTONICS4LIFE of FP7-ICT-2007-2, project №1.4.09 of RF Ministry of Education and Science, RF Governmental contracts 02.740.11.0770, 02.740.11.0879, 11.519.11.2035, 14.B37.21.0728, and 14.B37.21.0563; FiDiPro, TEKES Program (40111/11), Finland; SCOPES Project IZ74ZO_137423/1 of Swiss National Science Foundation; and 1177.2012.2 “Support for the Leading Scientific Schools” from the President of the RF.

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  1. Huazhong University of Science and Technology, Wuhan, 430074, People’s Republic of China

    Qingming Luo, Chao Jiang, Pengcheng Li, Haiying Cheng, Zhen Wang & Zheng Wang

  2. Department of Optics and Biophotonics, Saratov State University, Saratov, 410012, Russian Federation

    Valery V. Tuchin

  3. Precision Mechanics and Control, Institute of the Russian Academy of Sciences, Saratov, 410028, Russian Federation

    Valery V. Tuchin

  4. Optoelectronics and Measurement Techniques Laboratory, University of Oulu, Oulu, 90014, Finland

    Valery V. Tuchin

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  1. Qingming Luo
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  2. Chao Jiang
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    Valery V. Tuchin

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Luo, Q. et al. (2013). Laser Speckle Imaging of Cerebral Blood Flow. In: Tuchin, V. (eds) Handbook of Coherent-Domain Optical Methods. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-5176-1_5

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