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Clinical Flow Cytometry in Molecular Genetic Pathology

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Abstract

Flow cytometry is a powerful diagnostic tool most commonly used for immunophenotyping of hematopoietic neoplasms and primary/secondary immunodeficiencies. Other applications include cell sorting, detection of chromosomal abnormalities based on in situ hybridization or polymerase chain reaction, and evaluation of various cellular functions such as proliferation, apoptosis, calcium efflux, and phosphorylation. Flow cytometry measures physical and antigenic properties of particles including cells, chromosomes, and individual molecules. Owing to the availability of numerous antibodies and ever expanding array of fluorochromes, flow cytometry is capable of simultaneous evaluation of multiple parameters. In hematolymphoid malignancies, flow cytometry can be used at the time of the initial diagnosis and for follow-up and prognostication. The diagnosis is based on aberrant immunophenotypes and maturation patterns, different from those typically seen in normal hematopoietic populations. This chapter focuses on the diagnostic applications of flow cytometry and presents a current approach to sample preparation, selection of antibody panels, and essential aspects of analysis and interpretation. In addition, a complete review of immunophenotypic features of major entities of 2008 World Health Organization classification of hematolymphoid neoplasms is discussed.

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Author information

Authors and Affiliations

  1. Department of Pathology and Laboratory Medicine, Indiana University School of Medicine, Indianapolis, IN, USA

    Dr. Magdalena Czader MD, PhD

Authors
  1. Dr. Magdalena Czader MD, PhD
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Editor information

Editors and Affiliations

  1. School of Medicine, Dept. Pathology, Indiana University, N. University Blvd. 550, Indianapolis, 46202, Indiana, USA

    Liang Cheng

  2. Dept. Pathology, Mount Sinai School of Medicine, Gustave L. Levy Place 1, New York, 10029, New York, USA

    David Y. Zhang

  3. School of Medicine, Department of Pathology and, Indiana University, 635 Barnhill Drive, Indianapolis, 46202, Indiana, USA

    John N. Eble

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Czader, M. (2013). Clinical Flow Cytometry in Molecular Genetic Pathology. In: Cheng, L., Zhang, D., Eble, J. (eds) Molecular Genetic Pathology. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-4800-6_10

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  • DOI: https://doi.org/10.1007/978-1-4614-4800-6_10

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  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4614-4799-3

  • Online ISBN: 978-1-4614-4800-6

  • eBook Packages: MedicineReference Module Medicine

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