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The Analyses of Neurotransmitters, Other Neuroactive Substances, and Their Metabolites Using Mass Spectrometry

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Handbook of Neurochemistry and Molecular Neurobiology

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Abstract:

The precise analysis of neurotransmitters and other neuroactive substances requires sensitive and selective techniques capable of isolating specific signals originating from compounds of interest from a large background of other biological interferences. Mass spectrometry is capable of identifying mass signals that are characteristic of compounds of identified or predicted interest. By combining mass spectrometry with a separation procedure (gas chromatography, liquid chromatography or others) it is possible to develop an accurate quantitative technique that eliminates almost all potentially interfering signals. This chapter summarizes the ways in which mass spectrometry is used to analyze neuroactive compounds and how it is combined with separation methods to provide accurate, quantitative estimates of the concentrations of these compounds in biological or other preparations. The advantages and limitations of particular methods are discussed and an extensive list of references is provided.

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Abbreviations

APCI:

Atmospheric Pressure Chemical Ionization

API‐electrospray:

Atmospheric Pressure Ionization Electrospray

APPI:

Atmospheric Pressure Photoionization

CE:

Capillary Electrophoresis

CI:

Chemical Ionization

CID:

Collision Induced Fragmentation

DC:

Direct Current

ECNI:

Electron Capture Negative Ion Chemical Ionization

EI:

Electron Ionization

ESI:

Electrospray Ionization

FAB:

Fast Atom Bombardment

GC:

Gas Chromatography

HIC:

Hydrophobic Interaction Chromatography

HILIC:

Hydrophilic Interaction Chromatography

HPLC:

High Performance Liquid Chromatography

LC:

Liquid Chromatography

MALDI:

Matrix‐Assisted Laser Desorption/Ionization

MS:

Mass Spectrometry

MS/MS:

Tandem Mass Spectrometry

m/z:

Mass to Charge

PCI:

Positive Chemical Ionization

PFTBA:

Perfluorotributylamine

RF:

Radio Frequency

SIM:

Single Ion Mode

TOF:

Time of Flight

UV:

Ultraviolet

VIS:

Visible

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Acknowledgments

The authors are grateful to the Canadian Institutes for Health Research (CIHR) (Ms. G. Rauw) for their financial support. Dr. Sloley wishes to thank Dr. Y.K. Tam for permission to use Novokin Biotech Inc. facilities for production of this manuscript. Dr. Soheir Tawfik is thanked for technical assistance.

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  1. B. D. Sloley
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  2. G. Rauw
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  3. R. T. Coutts
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Editors and Affiliations

  1. Center for Neurochemistry, Nathan S. Kline Institute for Psychiatric Research, 140 Old Orangeburg Road, Orangeburg, New York, 10962, USA

    Abel Lajtha (Director) (Director)

  2. Department of Pharmacology, University of Alberta, Edmonton, Alberta, T6G 2H7, Canada

    Glenn Baker , Susan Dunn  & Andrew Holt ,  & 

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Sloley, B.D., Rauw, G., Coutts, R.T. (2007). The Analyses of Neurotransmitters, Other Neuroactive Substances, and Their Metabolites Using Mass Spectrometry. In: Lajtha, A., Baker, G., Dunn, S., Holt, A. (eds) Handbook of Neurochemistry and Molecular Neurobiology. Springer, New York, NY. https://doi.org/10.1007/978-0-387-30401-4_6

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