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Practical Enzymology

Quantifying Enzyme Activity and the Effects of Drugs Thereupon

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Abstract:

Neuroscientists may wish to quantify an enzyme activity for one of many reasons. In order to do so, the researcher must be able to set up an assay appropriately, and this requires some understanding of the kinetic behavior of the enzyme toward the substrate used. Furthermore, such an understanding is vital if the inhibitory effects of a drug are to be assessed appropriately. This chapter outlines key principles that must be adhered to, and describes basic approaches by which rather complex kinetic data might be obtained, in order that enzyme kinetics and inhibitor kinetics might be studied successfully by the nonexpert.

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Acknowledgments

Work in the author's laboratory is supported by the Canadian Institutes of Health Research (CIHR), the Faculty of Medicine and Dentistry of the University of Alberta, the Northern Alberta Clinical Trial and Research Centre, and BioTie Therapies Corp. (Turku, Finland).

Authors
  1. A. Holt
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Editors and Affiliations

  1. Center for Neurochemistry, Nathan S. Kline Institute for Psychiatric Research, 140 Old Orangeburg Road, Orangeburg, New York, 10962, USA

    Abel Lajtha (Director) (Director)

  2. Department of Pharmacology, University of Alberta, Edmonton, Alberta, T6G 2H7, Canada

    Glenn Baker , Susan Dunn  & Andrew Holt ,  & 

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© 2007 Springer Science+Business Media, LLC

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Holt, A. (2007). Practical Enzymology. In: Lajtha, A., Baker, G., Dunn, S., Holt, A. (eds) Handbook of Neurochemistry and Molecular Neurobiology. Springer, New York, NY. https://doi.org/10.1007/978-0-387-30401-4_4

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