Abstract:
Radiolabelled ligand binding studies remain one of the most widely used techniques in characterizing the biochemical and pharmacological properties of receptors. In this chapter, we provide an overview of radiolabelled ligand binding, discussing both theoretical and practical aspects of these experiments. Specific protocols that have been used in our laboratories to study the binding of radiolabelled ligands to two members of a ligandāgated ion channel family i.e., the nicotinic and GABAA receptors are included.
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Abbreviations
- ACh:
-
acetylcholine
- DNPP:
-
diethylāpānitrophenyl phosphate
- GABA:
-
Ī³āaminobutyric acid
- nAChR:
-
nicotinic acetylcholine receptor
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Martin, I.L., Davies, M., Dunn, S.M.J. (2007). Characterization of Receptors by Radiolabelled Ligand Binding Techniques. In: Lajtha, A., Baker, G., Dunn, S., Holt, A. (eds) Handbook of Neurochemistry and Molecular Neurobiology. Springer, New York, NY. https://doi.org/10.1007/978-0-387-30401-4_10
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DOI: https://doi.org/10.1007/978-0-387-30401-4_10
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