Electron Cryo-microscopy of Molecular Machines
- Junjie ZhangAffiliated withDepartment of Structural Biology, Stanford University Email author
- , Wah ChiuAffiliated withNational Center for Macromolecular Imaging, Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine
Molecular machine: A large complex made up of multiple copies of proteins and/or nucleic acids with a molecular size ranging from hundreds of kDa to tens of MDa. Examples of molecular machines are virus, ribosome, chaperonin, etc.
Electron cryo-microscopy (Cryo-EM): A structural method uses electron microscope to record images of frozen, hydrated specimens and uses computational procedures to reconstruct its three-dimensional density.
Single particle cryo-EM: A special application of cryo-EM where the specimen is a noncrystalline type embedded in a thin layer of vitreous ice as individual particles in random orientations.
Single-particle electron cryo-microscopy (cryo-EM) is an emerging structural biophysics technique for imaging individual molecular machines. Advantages of the cryo-EM imaging technique are that it can be used to d ...
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- Electron Cryo-microscopy of Molecular Machines
- Reference Work Title
- Encyclopedia of Biophysics
- pp 573-577
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- Online ISBN
- Springer Berlin Heidelberg
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- European Biophysical Societies' Association (EBSA)
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- Editor Affiliations
- 1. Department of Biochemistry, University of Leicester
- Author Affiliations
- 06191. Department of Structural Biology, Stanford University, Stanford, CA, 94305, USA
- 06192. National Center for Macromolecular Imaging, Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, One Baylor Plaza, Houston, TX, 77030, USA
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