- Dr. rer. nat. Manfred Schwab
- … show all 1 hide
Amplification is the selective increase of DNA copy number either intracellularly, as a local genomic change, or experimentally, by polymerase chain reaction (PCR). Increase of the level of mRNA or protein alone should not be referred to as amplification.
Intracellular amplification results in a selective increase in gene copy number with the consequence of elevated gene expression. Gene amplification has been seen in three different settings:
Scheduled amplification as part of a developmental gene expression program, e.g., chorion genes in ovaries of the fruit fly Drosophila melanogaster or actin genes during myogenesis in the chicken.
Unscheduled amplification during acquisition of cellular drug resistance. For example, amplification of the gene encoding dihydrofolate reductase (DHFR) can result in up to 1,000 gene copies per cell with the consequence of cellular resistance against the chemotherapy drug methotrexate
- Schwab M (1998) Amplification of oncogenes in human cancer cells. BioEssays 20:473–479 CrossRef
- Savelyeva L, Schwab M (2001) Amplification of oncogenes revisited: from expression profiling to clinical application. Cancer Lett 167:115–123 CrossRef
- Schwab M, Westermann F, Hero B et al (2003) Neuroblastoma: biology, and molecular and chromosomal pathology. Lancet Oncol 4:472–480 CrossRef
- Reference Work Title
- Encyclopedia of Cancer
- pp 160-163
- Print ISBN
- Online ISBN
- Springer Berlin Heidelberg
- Copyright Holder
- Springer-Verlag Berlin Heidelberg
- Additional Links
- Industry Sectors
- eBook Packages
To view the rest of this content please follow the download PDF link above.