- Manfred SchwabAffiliated withDKFZ Email author
Amplification is the selective increase of DNA copy number either intracellularly, as a local genomic change, or experimentally, by polymerase chain reaction (PCR). Increase of the level of mRNA or protein alone should not be referred to as amplification.
Scheduled amplification as part of a developmental gene expression program, e.g., chorion genes in ovaries of the fruit fly Drosophila melanogaster or actin genes during myogenesis in the chicken.
Unscheduled amplification during acquisition of cellular drug resistance. For example, amplification of the gene encoding dihydrofolate reductase (DHFR) can result in up to 1,000 gene copies per cell with the consequence of cellular resistance against the chemotherapy drug
- Reference Work Title
- Encyclopedia of Cancer
- pp 160-163
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- Springer Berlin Heidelberg
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- Springer-Verlag Berlin Heidelberg
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