- Haruyuki KamiyaAffiliated withDepartment of Neurobiology, Department of Molecular Neuroanatomy, Hokkaido University School of Medicine
Acute brain slice
Thin sections (∼400 μm thick) of part of the brain kept in oxygenated artificial cerebrospinal fluid.
In the mammalian central nervous system (CNS), pharmacological studies were seriously hampered by the presence of the blood brain barrier (BBB). Direct infusion of drugs into the small brain regions is technically difficult in vivo, and it is rather hard to estimate the actual concentration of the drug at the recording site. In order to develop alternative in vitro experimental systems enabling pharmacological studies in a quantitative manner, in the mid 1960s, Yamamoto and McIlwain, devised a novel way to keep intact neuronal networks in thin slices of anterior piriform cortex [1,2]. Afterwards, it was revealed that this method is applicable to many cortical areas including neocortex, cerebellum, hippocampus, etc (Figs. 1 and 2).
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- Reference Work Title
- Encyclopedia of Neuroscience
- pp 3743-3745
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- Springer Berlin Heidelberg
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- Springer-Verlag GmbH Berlin Heidelberg
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- Editor Affiliations
- 1. Department of Physiology & Biophysics, University of Washington School of Medicine
- 2. Department of Cell Biology and Anatomy, Graduate School of Medicine University of Tokyo Hongo
- Haruyuki Kamiya (1)
- Author Affiliations
- 1. Department of Neurobiology, Department of Molecular Neuroanatomy, Hokkaido University School of Medicine, Sapporo, Japan
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