Reference Work Entry

Encyclopedia of Genetics, Genomics, Proteomics and Informatics

pp 829-829

Guanidinium Isothiocyanate

Guanidinium isothiocyanate is used for the isolation of RNA. It breaks up cells, dissociates nucleoproteins, and inactivates tough RNase enzymes (at 4 M solutions) in the presence of the reducing agent β-mercaptoethanol. RNA extraction

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