Reference Work Entry

Encyclopedia of Genetics, Genomics, Proteomics and Informatics

pp 231-232

Date:

Bradford Method (Anal Biochem 72:248)

The Bradford protein assay, for 1 to 100 μg protein. Prepare a standard solution (0.5 mg/mL) of bovine serum albumin (BSA) and make a dilution series 5 to 20 μL and dilute to 100 μL with 0.15 M NaCl. Prepare also 0.15 M NaCl blanks. Make a series of dilutions also from the unknown quantity of the protein to be tested. Add 1 mL Coomassie brilliant blue to all and mix thoroughly. After 2 min determine absorption at 1‐cm path‐length at 595‐nm wavelength in a spectrophotometer and extrapolate the concentration of the sample from the standard series. Lowry test, Kjeldahl method

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