YAC

  • show all 0 hide
This is an excerpt from the content

Refers to yeast artificial chromosome vectors equipped with a yeast centromere and some (Tetrahymena) telomeres in a linear plasmid containing selectable markers, ARS (autonomously replicating sequence) for the maintenance and propagation of eukaryotic DNA inserts in cloning of larger than 200 kb size sequences.YACs play an important role in identifying contigs in physical mapping of larger genomes, as well as in in situ hybridization, map based gene isolation, etc. The rate of instability of YAC has been estimated to be about 2%. In mitotic yeast cells YACs behave like other chromosomes. Meiosis can be analyzed by tetrads although recombination appears to be reduced. YACs may be maintained through some cell divisions in the mouse cytoplasm and behave like double minute chromosomes or they may be integrated into the mouse chromosomes. anchoring, contigs, YAC library, in situ hybridization, chromosome walking, pulsed field gel electrophoresis, DM; Peterson KR 1999 Methods Enzymol 306:18 ...