Reference Work Entry

Encyclopedia of Genetics, Genomics, Proteomics and Informatics

pp 1388-1388

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O.D.

Optical density indicates the absorption of light at a particular wavelength by a compound in a spectrophotometer. O.D. can be used to characterize a molecule, e.g., pure nucleic acids have maximal absorption at 260 nm but contamination and the solvent may alter the absorption pattern. DNA measurements, extinction

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