Abstract
A comprehensive collection of different methods for extracting high-quality genomic DNA from Gram-positive and -negative bacteria and fungal mycelium and spores is described in this chapter. Special care has been taken in describing the ideal ratio of biological material to chemical reagents for an efficient extraction of genomic DNA, and in stating the appropriate application in molecular biology protocols (e.g., PCR or genomic DNA library-cloning quality).
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References
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© 2007 Humana Press Inc.
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Park, D. (2007). Genomic DNA Isolation From Different Biological Materials. In: Hilario, E., Mackay, J. (eds) Protocols for Nucleic Acid Analysis by Nonradioactive Probes. Methods in Molecular Biology, vol 353. Humana Press. https://doi.org/10.1385/1-59745-229-7:3
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DOI: https://doi.org/10.1385/1-59745-229-7:3
Publisher Name: Humana Press
Print ISBN: 978-1-58829-430-2
Online ISBN: 978-1-59745-229-8
eBook Packages: Springer Protocols