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Grapevine (Vitis vinifera L.)

  • Protocol
Agrobacterium Protocols Volume 2

Part of the book series: Methods in Molecular Biology ((MIMB,volume 344))

Abstract

Grapevine (Vitis) is considered to be one of the major fruit crops in the world based on hectares cultivated and economic value. Grapes are used not only for wine but also for fresh fruit, dried fruit, and juice production. Wine is by far the major product of grapes and the focus of this chapter is on wine grape cultivars. Grapevine cultivars of Vitis vinifera L. have a reputation for producing premium quality wines. These premium quality wines are produced from a small number of cultivars that enjoy a high level of consumer acceptance and are firmly entrenched in the market place because of varietal name branding and the association of certain wine styles and regions with specific cultivars. In light of this situation, grapevine improvement by a transgenic approach is attractive when compared to a classical breeding approach. The transfer of individual traits as single genes with a minimum disruption to the original genome would leave the traditional characteristics of the cultivar intact. However, a reliable transformation system is required for a successful transgenic approach to grapevine improvement. There are three criteria for achieving an efficient Agrobacterium-mediated transformation system: (1) the production of highly regenerative transformable tissue, (2) optimal co-cultivation conditions for both grapevine tissue and Agrobacterium, and (3) an efficient selection regime for transgenic plant regeneration. In this chapter, we describe a grapevine transformation system which meets the above mentioned criteria.

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References

  1. Mullins, M.G., Bouquet, A., and Williams, L.E. (1992) Biology of Grapevine. Cambridge University Press, Cambridge, UK.

    Google Scholar 

  2. Kikkert, J.R., Thomas, M.R., and Reisch, B.I. (2001) Grapevine genetic engineering. In: Molecular Biology and Biotechnology of the Grapevine (Roubelakis-Angelakis, K.A., ed.), Kluwer Academic Publishers, Dordrecht, The Netherlands, pp. 393–410.

    Google Scholar 

  3. Bouquet, A., Chatelet, P., and Torregrosa, L. (2003) Grapevine genetic engineering: tool for genome analysis or plant breeding method? Which future for transgenic vines? AgBiotechNet 5, ABN 116, 1–10.

    Google Scholar 

  4. Colova-Tsolova, V., Perl, A., Krastanova, S., Tsvetkov, I., and Atanassov, A. (2001) Genetically engineered grape for disease and stress tolerance. In: Molecular Biology and Biotechnology of the Grapevine (Roubelakis-Angelakis, K.A., ed.), Kluwer Academic Publishers, Dordrecht, The Netherlands, pp. 411–432.

    Google Scholar 

  5. Mullins, M.G., Tang, F.C.A., and Facciotti, D. (1990) Agrobacterium-mediated genetic transformation of grapevines. Transgenic plants of Vitis rupestris Scheele and buds of Vitis vinifera L. Nat. Biotechnol. 8, 1041–1045.

    Article  CAS  Google Scholar 

  6. Perl, A., Colova-Tsolova, V., and Eshdat, Y. (2004) Agrobacterium-mediated transformation of grape embryogenic calli. In: Transgenic Crops of the World (Curtis, I.S., ed.), Kluwer Academic Publishers, Dordrecht, The Netherlands, pp. 1–14.

    Google Scholar 

  7. Colby, S.M., Juncosa, A.M., and Meredith, C.P. (1991) Cellular differences in Agrobacterium susceptibility and regenerative capacity restrict the development of transgenic grapevines. J. Amer. Soc. Hort. Sci. 116, 356–361.

    Google Scholar 

  8. Mezzetti, B., Pandolfini, T., Navacchi, O., and Landi, L. (2002) Genetic transformation of Vitis vinifera via organogenesis. BMC Biotechnology 2, 18–28.

    Article  PubMed  Google Scholar 

  9. Martinelli, L. and Gribaudo, I. (2001) Somatic embryogenesis in grapevine. In: Molecular Biology and Biotechnology of the Grapevine (Roubelakis-Angelakis, K.A., ed.), Kluwer Academic Publishers, Dordrecht, The Netherlands, pp. 327–351.

    Google Scholar 

  10. Le Gall, O., Torregrosa, L., Danglot, Y., Candresse, T., and Bouquet, A. (1994) Agrobacterium-mediated genetic transformation of grapevine somatic embryos and regeneration of transgenic plants expressing the coat protein of grapevine chrome mosaic nepovirus (GCMV). Plant Science 102, 161–170.

    Article  Google Scholar 

  11. Perl, A., Lotan, O., Abu-Abied, M., and Holland, D. (1996) Establishment of an Agrobacterium-mediated transformation system for grape (Vitis vinifera L.): The role of antioxidants during grape-Agrobacterium interactions. Nat. Biotechnol. 14, 624–628.

    Article  PubMed  CAS  Google Scholar 

  12. Franks, T., Gang He, D., and Thomas, M., (1998) Regeneration of transgenic Vitis vinifera L. Sultana plants: genotypic and phenotypic analysis. Mol. Breeding 4, 321–333.

    Google Scholar 

  13. Reustle, G.M., Wallbraun, M., Zwiebel, M., et al. (2002) Experience with different selectable marker systems for the genetic engineering of grapevine. Acta Hort.. 603, 485–490.

    Google Scholar 

  14. Torregrosa, L., Péros, J.-P., Lopez, G., and Bouquet, A. (2000) Effect of hygromycin, kanamycin and phosphinothricin on the embryogenic development and axillary micropropagation of Vitis vinifera L. Acta Hort. 528, 401–406.

    CAS  Google Scholar 

  15. Kieffer, F., Triouleyre, C., Bertsch, C., Farine, S., Leva, Y., and Walter, B. (2004) Mannose and xylose cannot be used as selectable agents for Vitis vinifera L. Vitis 43, 35–39.

    CAS  Google Scholar 

  16. Hood, E.E., Gelvin, S.B., Melchers, L.S., and Hoekema, A. (1993) New Agrobacterium helper plasmids for gene transfer to plants. Transgenic Res. 2, 208–218.

    Article  CAS  Google Scholar 

  17. Torregrosa, L., Iocco, P., and Thomas, M.R. (2002) Influence of Agrobacterium strain, culture medium, and cultivar on the transformation efficiency of Vitis vinifera L. Am. J. Enol. Vitic. 53, 183–190.

    CAS  Google Scholar 

  18. Garfinkel, D.J. and Nester, E.W. (1980) Agrobacterium mutants affected in crown gall tumerorigenesis and octopine catabolism. J. Bacteriol. 144, 732–743.

    PubMed  CAS  Google Scholar 

  19. Chilton, M.D., Currier, T.C., Farrand, S.K., Bendish, A.J., Gordon, M.P., and Nester, E.W. (1974) Agrobacterium tumefaciens DNA and PS8 bacteriophage DNA not detected in crown gall tumors. Proc. Natl. Acad. Sci. USA 71, 3672–3676.

    Article  PubMed  CAS  Google Scholar 

  20. Nitsch, J.-P. and Nitsch, C. (1969) Haploid plants from pollen grains. Science 165, 85–87.

    Article  Google Scholar 

  21. Gamborg, O.L., Miller, R.A., and Ojima, K. (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell Res. 50, 1515–1518.

    Article  Google Scholar 

  22. Murashige, T. and Skoog, F. (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15, 473–497.

    Article  CAS  Google Scholar 

  23. Torregrosa, L., Torres-Viñals, M., and Bouquet, A. (1995) Somatic embryogenesis from leaves of Vitis x Muscadinia hybrids. Vitis 34, 239–240.

    Google Scholar 

  24. Torregrosa, L. (1998) A simple and efficient method to obtain stable embryogenic cultures from anthers of Vitis vinifera L. Vitis 37, 91–92.

    Google Scholar 

  25. Galzy, R., Haffner, V., and Compan, D. (1990) Influence of three factors on the growth and nutrition of grapevine microcuttings. J. Exp. Bot. 41, 295–301.

    Article  Google Scholar 

  26. Iocco, P., Franks, T., and Thomas, M.R. (2001) Genetic transformation of major wine grape cultivars of Vitis vinifera L. Transgenic Res. 10, 105–112.

    Article  PubMed  CAS  Google Scholar 

  27. Bowers, J., Boursiquot, J.-M., This, P., Chu, K., Johansson, H., and Meredith, C. (1999) Historical genetics: The parentage of Chardonnay, Gamay and other wine grapes of Northeastern France. Science 285, 1562–1565.

    Article  PubMed  CAS  Google Scholar 

  28. Mullins, M.G. (1966) Test-plants for investigations of physiology of fruiting in Vitis vinifera L. Nature 209, 419–420.

    Article  Google Scholar 

  29. Martinelli, L. and Mandolino, G. (2001) Transgenic grapes (Vitis species). In: Biotechnology in Agriculture and Forestry, Vol.47, Transgenic Crops II (Bajaj, Y.P.S., ed.), Springer-Verlag, Berlin, Germany, pp. 325–338.

    Google Scholar 

  30. Legrand, V., Dalmayrac, S., Latché, A., et al. (2003) Constitutive expression of Vr-ERE gene in transformed grapevines confers enhanced resistance to eutypine, a toxin from Eutypa lata. Plant Science 164, 809–814.

    Article  CAS  Google Scholar 

  31. Motoike, S.Y., Skirvin, R.M., Norton, M.A., and Otterbacher, A.G. (2002) Development of methods to genetically transform American grape (Vitis × labruscana L.H. Bailey). J. Hort. Sci. Biotech. 77, 691–696.

    Google Scholar 

  32. Mauro, M.-C., Toutain, S., Walter, B., et al. (1995) High efficiency regeneration of grapevine plants transformed with the GFLV coat protein gene. Plant Science 112, 97–106.

    Article  CAS  Google Scholar 

  33. Bouquet, A. and Torregrosa, L. (2003) Micropropagation of the grapevine. In: Micropropagation of Woody Trees and Fruits (Jain, S.M. and Ishii, K., eds.), Kluwer Academic Publishers, Dordrecht, The Netherlands, pp. 319–352.

    Google Scholar 

  34. Torregrosa, L., Bouquet, A., and Goussard, P.G. (2001) In vitro culture and propagation of grapevine. In: Molecular Biology and Biotechnology of the Grapevine (Roubelakis-Angelakis, K.A., eds.), Kluwer Academic Publishers, Dordrecht, The Netherlands, pp. 281–326.

    Google Scholar 

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Author information

Authors and Affiliations

  1. UMR Biology of Development of Cultivated Perennial Plants, Agro-M/INRA, 2, place Pierre Viala, 34060, Montpellier Cedex1, France

    Alain Bouquet & Laurent Torregrosa

  2. CSIRO Plant Industry, Horticulture Unit, PO Box 350, Glen Osmond, SA, 5064, Australia

    Pat Iocco & Mark R. Thomas

Authors
  1. Alain Bouquet
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  2. Laurent Torregrosa
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  3. Pat Iocco
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  4. Mark R. Thomas
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Editor information

Editors and Affiliations

  1. Center for Plant Transformation, Plant Science Institute, Iowa State Universtiy, Ames, Iowa

    Kan Wang

  2. Department of Agronomy, Iowa State Universtiy, Ames, Iowa

    Kan Wang

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© 2006 Humana Press Inc., Totowa, NJ

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Bouquet, A., Torregrosa, L., Iocco, P., Thomas, M.R. (2006). Grapevine (Vitis vinifera L.). In: Wang, K. (eds) Agrobacterium Protocols Volume 2. Methods in Molecular Biology, vol 344. Humana Press. https://doi.org/10.1385/1-59745-131-2:273

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  • DOI: https://doi.org/10.1385/1-59745-131-2:273

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-843-0

  • Online ISBN: 978-1-59745-131-4

  • eBook Packages: Springer Protocols

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