Protocol

Agrobacterium Protocols Volume 2

Volume 344 of the series Methods in Molecular Biology pp 13-24

Cassava (Manihot esculenta Crantz)

  • Weston MsikitaAffiliated withDepartment of Molecular Genetics and Cell Biology, University of Chicago
  • , Uzoma IhemereAffiliated withDepartment of Plant Cellular and Molecular Biology, The Ohio State University
  • , Dimuth SiritungaAffiliated withDepartment of Biology, University of Puerto Rico
  • , Richard T. SayreAffiliated withDepartment of Plant Cellular and Molecular Biology, The Ohio State University

* Final gross prices may vary according to local VAT.

Get Access

Abstract

During the last three years the generation of stably transformed cassava plants having value-added traits has become a reality. Currently, two Agrobacterium-mediated transformation systems are routinely used to engineer cassava. These systems use either somatic embryos or friable embryogenic calli. This paper presents detailed protocols for the transformation of cassava using primary somatic embryos. The effects of explant types, tissue culture conditions, and bacterial and plasmid related factors on transformation efficiency are discussed.

Key Words

Agrobacterium tumefaciens apical leaves auxiliary buds cassava direct embryogenesis explant friable embryogenic callus genetic transformation germinating somatic embryos Manihot esculenta Crantz shoot regeneration somaclonal variation