Protocol

Agrobacterium Protocols Volume 2

Volume 344 of the series Methods in Molecular Biology pp 351-358

Rose (Rosa hybrida L.)

  • Schuyler S. KorbanAffiliated withDepartment of Natural Resources & Environmental Sciences, University of Illinois
  • , Ksenija GasicAffiliated withDepartment of Natural Resources & Environmental Sciences, University of Illinois
  • , Xiangqian LiAffiliated withDivision of Biological Sciences, University of California-San Diego

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Abstract

Although rose transformation is successful, it remains difficult to transform myriad rose species as well as different rose genotypes. In this protocol, a detailed description of rose transformation is presented. This protocol relied on Agrobacterium-mediated transfer of embryogenic callus cultures. There are many critical steps that must be followed to achieve successful transformation; however, it is important to keep in mind that these apply to a selected number of genotypes, and as a different genotype is subjected to transformation, modifications of this protocol must be made to achieve successful transformation.

Key Words

Agrobacterium tumefaciens Rosa hybrida genetic transformation