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Rose (Rosa hybrida L.)

  • Protocol
Agrobacterium Protocols Volume 2

Part of the book series: Methods in Molecular Biology ((MIMB,volume 344))

Abstract

Although rose transformation is successful, it remains difficult to transform myriad rose species as well as different rose genotypes. In this protocol, a detailed description of rose transformation is presented. This protocol relied on Agrobacterium-mediated transfer of embryogenic callus cultures. There are many critical steps that must be followed to achieve successful transformation; however, it is important to keep in mind that these apply to a selected number of genotypes, and as a different genotype is subjected to transformation, modifications of this protocol must be made to achieve successful transformation.

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Author information

Authors and Affiliations

  1. Department of Natural Resources & Environmental Sciences, University of Illinois, 310 ERML, Urbana, IL

    Schuyler S. Korban & Ksenija Gasic

  2. Division of Biological Sciences, University of California-San Diego, La Jolla, CA

    Xiangqian Li

Authors
  1. Schuyler S. Korban
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  2. Ksenija Gasic
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  3. Xiangqian Li
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Editor information

Editors and Affiliations

  1. Center for Plant Transformation, Plant Science Institute, Iowa State Universtiy, Ames, Iowa

    Kan Wang

  2. Department of Agronomy, Iowa State Universtiy, Ames, Iowa

    Kan Wang

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© 2006 Humana Press Inc., Totowa, NJ

About this protocol

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Korban, S.S., Gasic, K., Li, X. (2006). Rose (Rosa hybrida L.). In: Wang, K. (eds) Agrobacterium Protocols Volume 2. Methods in Molecular Biology, vol 344. Humana Press. https://doi.org/10.1385/1-59745-131-2:351

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  • DOI: https://doi.org/10.1385/1-59745-131-2:351

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-843-0

  • Online ISBN: 978-1-59745-131-4

  • eBook Packages: Springer Protocols

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