A New Automated Fourth-Generation HIV Screening Assay With Sensitive Antigen Detection Module and High Specificity
- Bernard Weber
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New screening enzyme immunoassays, which permit the simultaneous detection of HIV antigens reduce the diagnostic window period between the time of immunodeficiency virus (HIV) infection and seroconversion. The VIDAS HIV DUO Ultra is an enzyme-linked fluorescent assay (ELFA) for the screening of HIV infection. It is performed with the fully automated VIDAS or mini-VIDAS instruments, which are so-called walk away systems. The detection limit is 3 pg of HIV-1 p24 Ag/mL serum. HIV antibody is detected with the same sensitivity as stand-alone third-generation antibody tests. The total incubation time is about 2 h. Results are calculated, interpreted, and printed by the VIDAS instrument. Usually, fourth-generation assays demand a special algorithm for the analysis of reactive samples. For the anti-HIV part of the assay, confirmation of reactivity should be done with an assay that lacks the p24-antigen detection module and when reactivity persists subsequently by immunoblot. For the p24-antigen part, confirmation of reactivity should be analyzed in an assay that lacks the anti-HIV detection part.
- Weber, B., Fall, E. M. B., Berger, A., and Doerr, H. W. (1998) Reduction of diagnostic window by new fourth-generation human immunodeficiency virus screening assays. J. Clin. Microbiol. 36, 2235–2239.
- Gürtler, L., Mühlbacher, A., Michl, U., Hofmann, H., Paggi, G., Bossi, V., et al. (1998) Reduction of the diagnostic window with a new combined p24 antigen and human immunodeficiency virus antibody screening assay. J. Virol. Meth. 75, 27–38. CrossRef
- Weber, B., Berger, A., Rabenau, H., and Doerr, H. W. (2002) Evaluation of a new combined antigen and antibody human immunodeficiency virus screening assay, VIDAS HIV DUO Ultra. J. Clin. Microbiol. 40, 1420–1426. CrossRef
- Weber, B., Gurtler, L., Thorstensson, R., Michl, U., Muhlbacher, A., Burgisser, P., et al. (2002) Multicenter evaluation of a new automated fourth-generation human immunodeficiency virus screening assay with a sensitive antigen detection module and high specificity. J. Clin. Microbiol. 40, 1938–1946. CrossRef
- Weber, B., Thorstensson, R., Tanprasert, S., Schmitt, U., and Melchior, W. (2003) Reduction of the diagnostic window in three cases of human immunodeficiency — 1 subtype E primary infection with fourth-generation HIV screening assays. Vox Sanguinis 85, 73–79. CrossRef
- Weber, B. (2003) HIV seroconversion: performance of combined antigen/antibody assays. AIDS 17, 931–945. CrossRef
- Ly, T. D., Edlinger, C., and Vabret, A. (2000) Contribution of combined detection assays of p24 antigen and anti-human immunodeficiency virus (HIV) antibodies in diagnosis of primary HIV infection by routine testing. J. Clin. Microbiol. 38, 2459–2461.
- Laboratory Bio Safety Manual, 2nd ed., 1993, WHO, Geneva, Switzerland.
- Saville, R. D., Constantine, N. T., Cleghorn, F. R., Jack, N., Bartholomew, C., Edwards, J., et al. (2001) Fourth-generation enzyme linked immunosorbent assay for the simultaneous detection of human immunodeficiency virus antigen and antibody. J. Clin. Microbiol. 39, 2518–2524. CrossRef
- A New Automated Fourth-Generation HIV Screening Assay With Sensitive Antigen Detection Module and High Specificity
- Book Title
- Human Retrovirus Protocols
- Book Subtitle
- Virology and Molecular Biology
- Book Part
- pp 245-255
- Print ISBN
- Online ISBN
- Series Title
- Methods in Molecular Biology™
- Series Volume
- Series ISSN
- Humana Press
- Copyright Holder
- Humana Press
- Additional Links
- Primary infection
- enzyme immunoassay (EIA)
- automated assay
- HIV screening
- Industry Sectors
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