Abstract
With the advent of proteomics, many previously unidentified proteins will be isolated in gels for subsequent structural and functional characterization. It is, therefore, important that methods are available for detecting these proteins with minimal risk of modification. This chapter describes a reverse staining technique that facilitates the sensitive detection of unmodified proteins (1–9).
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© 2005 Humana Press Inc., Totowa, NJ
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Fernandez-Patron, C. (2005). Zn2+Reverse Staining Technique. In: Walker, J.M. (eds) The Proteomics Protocols Handbook. Springer Protocols Handbooks. Humana Press. https://doi.org/10.1385/1-59259-890-0:185
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DOI: https://doi.org/10.1385/1-59259-890-0:185
Publisher Name: Humana Press
Print ISBN: 978-1-58829-343-5
Online ISBN: 978-1-59259-890-8
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