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DNA Viruses pp 161–172Cite as

FTIR Microscopy Detection of Cells Infected With Viruses

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 292))

Summary

Fourier-transform infrared (FTIR) microscopy is considered a comprehensive and sensitive method for detection of molecular changes in cells. The advantage of FTIR microspectroscopy over conventional FTIR spectroscopy is that it facilitates inspection of restricted regions of a cell culture or a tissue. We have shown that it is possible to apply FTIR microscopy as a sensitive and effective assay for the detection of cells infected with various members of the herpes family of viruses and retroviruses. Detectable and significant spectral differences between normal and infected cells were evident at early stages of the infection. Impressive changes in several spectroscopic parameters were seen in infected compared with uninfected cells. It seems that the change in spectral behavior is specific to the infecting virus, because cells infected with herpesviruses showed different spectral changes compared with cells infected with retoviruses.

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Author information

Authors and Affiliations

  1. The Institute for Applied Biosciences, Ben-Gurion University of the Negev, Beer-Sheva, Israel

    Vitaly Erukhimovitch, Marina Talyshinsky, Yelena Souprun & Mahmoud Huleihel

Authors
  1. Vitaly Erukhimovitch
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  2. Marina Talyshinsky
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  3. Yelena Souprun
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  4. Mahmoud Huleihel
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Editor information

Editors and Affiliations

  1. The Wistar Institute, Philadelphia, PA

    Paul M. Lieberman

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© 2005 Humana Press Inc.

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Erukhimovitch, V., Talyshinsky, M., Souprun, Y., Huleihel, M. (2005). FTIR Microscopy Detection of Cells Infected With Viruses. In: Lieberman, P.M. (eds) DNA Viruses. Methods in Molecular Biology, vol 292. Humana Press. https://doi.org/10.1385/1-59259-848-X:161

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  • DOI: https://doi.org/10.1385/1-59259-848-X:161

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-353-4

  • Online ISBN: 978-1-59259-848-9

  • eBook Packages: Springer Protocols

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