Protocol

Apoptosis Methods and Protocols

Volume 282 of the series Methods in Molecular Biology pp 207-221

Measurement of Apoptotic Cell Clearance In Vitro

  • Andrew DevittAffiliated withCentre for Inflammation Research, The University of Edinburgh Medical School
  • , Christopher D. GregoryAffiliated withCentre for Inflammation Research, The University of Edinburgh Medical School

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Abstract

The phagocytic clearance of apoptotic cells is a highly efficient and non-phlogistic process in vivo. Research in this area has been limited, at least in part, by technical difficulties associated with the techniques used in the detailed study of apoptotic cell clearance mechanisms. This chapter provides details of methods that may be used to study apoptotic cell clearance in vitro. Such methods have been used successfully to identify phagocyte-associated or apoptotic cell-associated molecular players in the recognition process.

Key Words

Macrophage phagocyte apoptotic cell clearance phagocytosis