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Public Health Microbiology pp 189–197Cite as

Immunomagnetic Separation of Pathogenic Organisms From Environmental Matrices

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 268))

Abstract

One of the most difficult challenges in the analysis of environmental samples is to separate the organism of interest from a sample that is high in background debris. Immunomagnetic separation (IMS) is one technique that has been developed to accomplish this in a rapid and reliable assay.

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References

  1. Dynal A. S. (1998) Dynabeads® Biomagnetic Applications in Cellular Immunology. Dynal, Oslo.

    Google Scholar 

  2. Jakobsen, K. S., Haugen, M., Sæbøe-Larssen, S., Hollung, K., Espelund, M., and Hornes, E. (1994) Direct mRNA isolation using magnetic oligo (dT) beads: a protocol for all types of cell cultures, animal and plant tissues. In: Advances in Biomagnetic Separation (Uhlén, M., Hornes, E., and Olsvik, O., eds.). Eaton, Natick, MA, pp. 61–72.

    Google Scholar 

  3. Korn, B., Sedlacek, Z., and Poustka, A. (1994) Isolation of transcribed and conserved sequences from large genomic regions by magnetic capture. In: Advances in Biomagnetic Separation (Uhlén, M., Hornes, E., and Olsvik, O., eds.). Eaton, Natick, MA, pp. 79–90.

    Google Scholar 

  4. Tagle, D. A., Swaroop, M., Elmer, L., et al. (1994) Magnetic bead capture of cDNAs: a startegy for isolating expressed sequences encoded within large genomic segments. In: Advances in Biomagnetic Separation (Uhlén, M., Hornes, E., and Olsvik, O., eds.). Eaton, Natick, MA, pp. 91–106.

    Google Scholar 

  5. Mörl, M., Dorner, M., and Pääbo, S. (1994) Direct purification of tRNAs using oligonucleotides coupled to magnetic beads. In: Advances in Biomagnetic Separation (Uhlén, M., Hornes, E., and Olsvik, O., eds.). Eaton, Natick, MA, pp. 107–112.

    Google Scholar 

  6. Mizutani, S., Asada, M., Wada, H., Yamada, A., and Kodama, C. (1994) Magnetic separation in molecular studies of human leukemia. In: Advances in Biomagnetic Separation (Uhlén, M., Hornes, E., and Olsvik, O., eds.). Eaton, Natick, MA, pp. 127–134.

    Google Scholar 

  7. Lawn, S. D., Roberts, B. D., Griffin, G., Folks, T., and Butera, S. (2000) Cellular compartments of human immunodeficiency virus type 1 replication in vivo: determination by presence of virion-associated host proteins and impact of opportunistic infection. J. Virol. 74, 139–194.

    Article  PubMed  CAS  Google Scholar 

  8. Rossomando, E. F. and White, L. (1994) In situ immunomagnetic capture of proteins from body fluids. In: Advances in Biomagnetic Separation (Uhlén, M., Hornes, E., and Olsvik, O., eds.). Eaton, Natick, MA, pp. 187–194.

    Google Scholar 

  9. Zhong, X. Y., Kaul, S., and Lin, Y. S. (2000) Sensitive detection of micrometastases in bone marrow from patients with breast cancer using immunomagnetic isolation of tumor cells in combination with reverse transcriptase/polymerase chain reaction for cytokeratin-19. J. Cancer Res. Clin. Oncol. 126, 212–218.

    Article  PubMed  CAS  Google Scholar 

  10. Calenda, V., Bolmont, C., and Chermann, J.-C. (1994) Immunomagnetic bone marrow cells separation: a rapid and efficient negative/positive cell selection method. In: Advances in Biomagnetic Separation (Uhlén, M., Hornes, E., and Olsvik, O., eds.). Eaton, Natick, MA, pp. 205–209.

    Google Scholar 

  11. Wang, J. E., Jørgensen, P. F., Almlöf, M., et al. (2000) Peptidoglycan and lipoteichoic acid from Staphylococcus aureus induce tumor necrosis factor alpha, interleukin 6 (IL-6), and IL-10 production in both T cells and monocytes in human whole blood model. Infect. Immun. 68, 3965–3970.

    Article  PubMed  CAS  Google Scholar 

  12. Bergquam, E., Avery, N., Shiigi, S., Axthelm, M., and Wong, S. (1999) Rhesus rhadinovirus establishes a latent infection in B lymphocytes in vivo. J. Virol. 73, 7874–7876.

    PubMed  CAS  Google Scholar 

  13. Sleasman, J. W., Leon, B., Aleixo, L., Rojas, M., and Goodenow, M. (1997). Immunomagnetic selection of purified monocyte and lymphocyte populations from peripheral blood mononuclear cells following cryopreservation. Clin. Diagn. Lab. Immunol. 4, 653–658.

    PubMed  CAS  Google Scholar 

  14. Islam, D. and Lindberg, A. (1992) Detection of Shigella dysenteriae type 1 and Shigella flexneri in feces by immunomagnetic isolation and polymerase chain reaction. J. Clin. Microbiol. 30, 2801–2806.

    PubMed  CAS  Google Scholar 

  15. Karch, H., Janetzki-Mittmann, C., Aleksic, S., and Datz, M. (1996) Isolation of enterohemorrhagic Escherichia coli 0157 strains from patients with hemolytic-uremic syndrome by using immunomagnetic separation, DNA-based methods, and direct culture. J. Clin. Microbiol. 34, 516–519.

    PubMed  CAS  Google Scholar 

  16. Li, Z., Bai, G. H., Fordham von Reyn, C., et al. (1996). Rapid detection of Mycobacterium avium in stool samples from AIDS patients by immunomagnetic PCR. J. Clin Microbiol. 34, 1903–1907.

    PubMed  CAS  Google Scholar 

  17. Mazurek, G. H., Reddy, V., Murphy, D., and Ansari, T. (1996) Detection of Mycobacterium tuberculosis in cerebrospinal fluid following immunomagnetic enrichment. J. Clin. Microbiol. 34, 450–453.

    PubMed  CAS  Google Scholar 

  18. Stark, M., Reizenstein, E., Uhlén, M., and Lundeberg, J. (1996) Immunomagnetic separation and solid-phase detection of Bordetella pertussis. J. Clin. Microbiol. 34, 778–784.

    PubMed  CAS  Google Scholar 

  19. Periera, M. G. C., Atwill, E., and Jones, T. (1999) Comparison of sensitivity of immunofluorescent microscopy to that of a combination of immunofluorescent microscopy and immunomagnetic separation for detection of Cryptosporidium parvum oocysts in adult bovine feces. Appl. Environ. Microbiol. 65, 3236–3239.

    Google Scholar 

  20. Deng, M. Q. and Cliver, D. O. (1998) Differentiation of Cryptosporidium parvum isolates by a simplified randomly amplified polymorphic DNA technique. Appl. Environ. Microbiol. 64, 1954–1957.

    PubMed  CAS  Google Scholar 

  21. Deng, M. Q., Cliver, D.O., and Mariam, T. W. (1997) Immunomagnetic capture PCR to detect viable Cryptosporidium parvum oocysts from environmental samples. Appl. Environ. Microbiol. 63, 3134–3138.

    PubMed  CAS  Google Scholar 

  22. Oberst, R. D., Hays, M. P., Bohra, L. K., et al. (1998) PCR based DNA amplification and presumptive detection of Escherichia coli O157:H7 with an internal fluorogenic probe and the 5′ nuclease (TaqMan) assay. Appl. Environ. Microbiol. 64, 3389–3396.

    PubMed  CAS  Google Scholar 

  23. Chapman, P. A., Cerdan Malo, A. T., Siddons, C. A., and Harkin, M. (1997) Use of commercial enzyme immunoassays and immunomagnetic separation systems for detecting Escherichia coli 0157 in bovine fecal samples. Appl. Environ. Microbiol. 63, 2549–2553.

    PubMed  CAS  Google Scholar 

  24. Tomoyasu, T. (1998) Improvement of the immunomagnetic separation method selective for Escherichia coli 0157 strains. Appl. Environ. Microbiol. 64, 376–382.

    PubMed  CAS  Google Scholar 

  25. Tsai, W. L., Miller, C. E., and Richter, E. R. (2000) Determination of the sensitivity of a rapid Escherichia coli O157:H7 assay for testing 375-gram composite samples. Appl. Environ. Microbiol. 66, 4149–4151.

    Article  PubMed  CAS  Google Scholar 

  26. Pyle, B. H., Broadaway, S. C., and McFeters, G. A. (1999) Sensitive detection of Escherichia coli O157:H7 in food and water by immunomagnetic separation and solid-phase laser cytometry. Appl. Environ. Microbiol. 65, 1966–1972.

    PubMed  CAS  Google Scholar 

  27. Yu, H. and Bruno, J. G. (1996) Immunomagnetic-electrochemiluminescent detection of Escherichia coli O157 and Salmonella typhimurium in foods and environmental water samples. Appl. Environ. Microbiol. 62, 587–592.

    PubMed  CAS  Google Scholar 

  28. Hanai, K., Satake, M., Nakanishi, H., and Venkateswaran, K. (1997) Comparison of commercially available kits with standard methods for detection of Salmonella strains in foods. Appl. Environ. Microbiol. 63, 775–778.

    PubMed  CAS  Google Scholar 

  29. Favrin, S. J., Jassim, S. A., and Griffiths, M. W. (2001) Development and optimization of a novel immunomagnetic separation-bacteriophage assay for detection of Salmonella enterica serovar enteritidis in broth. Appl. Environ. Microbiol. 67, 217–224.

    Article  PubMed  CAS  Google Scholar 

  30. Roberts, B. and Hirst, R. (1997) Immunomagnetic separation and PCR for detection of Mycobacterium ulcerans. J. Clin. Microbiol. 35, 2709–2711.

    PubMed  CAS  Google Scholar 

  31. Grant, I. R., Ball, H. J., and Rowe, M. T. (1998) Isolation of Mycobacterium paratuberculosis from milk by immunomagnetic separation. Appl. Environ. Microbiol. 64, 3153–3158.

    PubMed  CAS  Google Scholar 

  32. Osaki, T., Taguchi, H., Yamaguchi, H., and Kamiya, S. (1998) Detection of Helicobacter pylori in fecal samples of gnotobiotic mice infected with H. pylori by an immunomagneticbead separation technique. J. Clin. Microbiol. 36, 321–323.

    PubMed  CAS  Google Scholar 

  33. Enroth, H. and Engstrand, L. (1995) Immunomagnetic separation and PCR detection of Helicobacter pylori in water and stool specimens. J. Clin. Microbiol. 33, 2162–2165.

    PubMed  CAS  Google Scholar 

  34. Tomoyasu, T. (1992) Development of the immunomagnetic enrichment method selective for Vibrio parahaemolyticus serotype K and its application to food poisoning study. Appl. Environ. Microbiol. 58, 2679–2682.

    PubMed  CAS  Google Scholar 

  35. Vuddhakul, V., Chowdhury, A., Laohaprertthisan, V., et al. (2000) Isolation of a pandemic O3:K6 clone of a vibrio parahaemolyticus strain from environmental and clinical sources in Thailand. Appl. Environ. Microbiol. 66, 2685–2689.

    Article  PubMed  CAS  Google Scholar 

  36. Dowd, S. E., Gerba, C. P., Enriquez, F. J., and Pepper, I. L. (1998) PCR amplification and species determination of microsporidia in formalin-fixed feces after immunomagnetic separation. Appl. Environ. Microbiol. 64, 333–336.

    PubMed  CAS  Google Scholar 

  37. Kapperud, G., Vardund, T., Skjerve, E., Hornes, E., and Michaelsen, T. E. (1993) Detection of pathogenic Yersinia enterocolitica in foods and water by immunomagnetic separation, nested polymerase chain reactions, and colorimetric detection of amplified DNA. Appl. Environ. Microbiol. 59, 2938–2944.

    PubMed  CAS  Google Scholar 

  38. Blake, M. R. and Weimer, B. C. (1997) Immunomagnetic detection of Bacillus stearothermophilus spores in food and environmental samples. Appl. Environ. Microbiol. 63: 1643–1646.

    PubMed  CAS  Google Scholar 

  39. Bruno, J. G. and Yu, H. (1996) Immunomagnetic-electrochemiluminescent detection of Bacillus anthracis spores in soil matrices. Appl. Environ. Microbiol. 62, 3474–3476.

    PubMed  CAS  Google Scholar 

  40. Muramatsu, Y., Yanase, T., Okabayashi, T., Ueno, H., and Morita, C. (1997) Detection of Coxiella burnetii in cow’s milk by PCR-enzyme-linked immunosorbent assay combined with a novel sample preparation method. Appl. Environ. Microbiol. 63, 2142–2146.

    PubMed  CAS  Google Scholar 

  41. Jothikumar, N., Cliver, D. O., and Mariam, T. W. (1998) Immunomagnetic capture PCR for rapid concentration and detection of hepatitus a virus from environmental samples. Appl. Environ. Microbiol. 64, 504–508.

    PubMed  CAS  Google Scholar 

  42. Hutchison, K. A., Perfect, S. E., O’Connell, R. J., and Green, J. R. (2000) Immunomagnetic purification of Colletotrichum lindemuthianum appressoria. Appl. Environ. Microbiol. 66, 3464–3467.

    Article  PubMed  CAS  Google Scholar 

  43. Biswas, B., Vemulapalli, R., and Dutta, S. K. (1994) Detection of Ehrlichia risticii from feces of infected horses by immunomagnetic separation and PCR. J. Clin. Microbiol. 32, 2147–2151.

    PubMed  CAS  Google Scholar 

  44. Christensen, B., Torsvik, T., and Lien, T. (1992) Immunomagnetically captured thermophilic sulfate-reducing bacteria from North Sea oil field waters. Appl. Environ. Microbiol. 58, 1244–1248.

    PubMed  CAS  Google Scholar 

  45. Fayer, R., Speer, C. A., and Dubey, J. P. (1997) The general biology of Cryptosporidium. In: Cryptosporidium and Cryptosporidiosis (Fayer, R. ed.). CRC, Boca Raton, FL, pp. 1–42.

    Google Scholar 

  46. Current, W. L., Reese, N. C., Erns, J. V. D. Bailey, W. S., Heyman, M. B., and Weistein, W. M. (1983) Human cryptosporidiosis in immunocompetant and immunodeficient persons: studies of an outbreak and experimental transmission. N. Engl. J. Med. 308, 1252–1257.

    Article  PubMed  CAS  Google Scholar 

  47. Dupont, H. L., Chappel, C. L., Sterling, C. R., Okhuysen, P. C., Rose, J. B., and Jakubowski, F. (1995) The infectivity of Cryptosporidium parvum in healthy volunteers. N. Engl. J. Med. 332, 855–859.

    Article  PubMed  CAS  Google Scholar 

  48. Casemore, D. P., Wright, S. E., and Coop, R. L. (1997) Cryptosporidiosis—human and animal epidemiology. In: Cryptosporidium and Cryptosporidiosis (Fayer, R., ed.). CRC, Boca Raton, FL, pp. 65–92.

    Google Scholar 

  49. Rose, J. B. (1997) Environmental ecology of Cryptosporidium and public health implications. Ann. Rev. Public Health 18, 135–161.

    Article  CAS  Google Scholar 

  50. Mackenzie, W., Neil, M., Hoxie, N., et al. (1994) A massive outbreak in Milwaukee of Cryptosporidium infection transmitted through the public water supply. N. Engl. J. Med. 331, 161–167.

    Article  CAS  Google Scholar 

  51. United States Environmental Protection Agency (1999) Method 1623: Cryptosporidium and Giardia in Water by Filtration/IMS/FA. EPA821-R-99-006. EPA, Washington, DC.

    Google Scholar 

  52. Bukhari, Z., McCuin, R. M., Fricker, C. R, and Clancy, J. L. (1998) Immunomagnetic separation of Cryptosporidium parvum from source water samples of various turbidities. Appl. Environ. Microbiol. 64, 4495–4499.

    PubMed  CAS  Google Scholar 

  53. Rochelle, P. A., De Leon, R., Johnson, A., Stewart, M. H., and Wolfe, R. L. (1999) Evaluation of immunomagnetic separation for recovery of infectious Cryptosporidium parvum oocysts from environmental samples. Appl. Environ. Microbiol. 65, 841–845.

    PubMed  CAS  Google Scholar 

  54. Dynal. (2000) GC Combo Kit Product Literature. Revision 06. Dynal, Oslo, Norway.

    Google Scholar 

  55. Yakub, G. P. and Stadterman-Knauer, K. L. (2000) Evaluation of immunomagnetic separation for recovery of Cryptosporidium parvum and Giardia duodenalis from high-iron matrices. Appl. Environ. Microbiol. 66, 3628–3631.

    Article  PubMed  CAS  Google Scholar 

  56. Di Giovanni, G. D., Hashemi, F. H., Shaw, N. J., Abrams, F. A., LeChevallier, M. W., and Abbaszadegan, M. (1999) Detection of infectious Cryptosporidium parvum oocysts in surface and filter backwash water samples by immunomagnetic separation and integrated cell culture-PCR. Appl. Environ. Microbiol. 65, 3427–3432.

    PubMed  Google Scholar 

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Author information

Authors and Affiliations

  1. North Huntington, PA

    Gary P. Yakub

  2. The Knauer Group, Pittsburgh, PA

    Kathleen L. Stadterman-Knauer

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  1. Gary P. Yakub
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  2. Kathleen L. Stadterman-Knauer
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Editors and Affiliations

  1. Planta Piloto de Procesos Industriales Microbiológicos, (PROIMI)-CONICET, San Miguel de Tucumán, Argentina

    John F. T. Spencer  & Alicia L. Ragout de Spencer  & 

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© 2004 Humana Press Inc.

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Yakub, G.P., Stadterman-Knauer, K.L. (2004). Immunomagnetic Separation of Pathogenic Organisms From Environmental Matrices. In: Spencer, J.F.T., Ragout de Spencer, A.L. (eds) Public Health Microbiology. Methods in Molecular Biology, vol 268. Humana Press. https://doi.org/10.1385/1-59259-766-1:189

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  • DOI: https://doi.org/10.1385/1-59259-766-1:189

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-117-2

  • Online ISBN: 978-1-59259-766-6

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