Abstract
Primer extension is often used to map the 5′ end of RNA (1). A single-stranded, end-labeled DNA primer is hybridized to RNA first. Using an RNA-dependent DNA polymerase (reverse transcriptase [RT]) and nonradioactive deoxynucleotides, the primer is extended to yield cDNA. The cDNA is then analyzed on a sequencing gel to determine nucleotide length. The length of the cDNA reflects the distance between the primer and the 5′ end of RNA, and hence, maps the transcription start sites.
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References
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© 2000 Humana Press Inc., Totowa, NJ
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Tseng, YT., Padbury, J.F. (2000). Primer Extension Methods for Determination of β1-Adrenergic Receptor mRNA Start Sites. In: Machida, C.A. (eds) Adrenergic Receptor Protocols. Methods in Molecular Biology™, vol 126. Humana Press. https://doi.org/10.1385/1-59259-684-3:181
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DOI: https://doi.org/10.1385/1-59259-684-3:181
Publisher Name: Humana Press
Print ISBN: 978-0-89603-602-4
Online ISBN: 978-1-59259-684-3
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