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Suicide Gene Therapy pp 263–278Cite as

Enzyme-Prodrug Systems

Thymidine Phosphorylase/5′-Deoxy-5-Fluorouridine

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Part of the book series: Methods in Molecular Medicine™ ((MIMM,volume 90))

Abstract

Thymidine phosphorylase (E.C. 2.4.2.4) (TP), also described as the angiogenic platelet-derived endothelial cell growth factor (PD-ECGF) (1–4), is a homodimeric enzyme with a monomeric molecular mass of about 55 kDa (5,6) that phosphorolytically cleaves thymidine to yield thymine and deoxyribose-1-phosphate (dR-1-P) (7,8). TP is expressed in various human cells and tissues and plays a role in plasma thymidine homeostasis (9–11). The levels of expression in different human tissues can vary up to 15-fold (12). Moreover, TP levels are increased in several types of malignant tumors when compared to the non-neoplastic regions of these tissues (13) and also in the plasma from tumor-bearing animals and cancer patients (14).

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Author information

Authors and Affiliations

  1. Department of Toxicology, Faculty of Pharmacy, University of Montpellier, Montpellier, France

    Alexandre Evrard, Pierre Cuq & Jean-Paul Cano

  2. Department of Pharmacokinetics and Toxicokinetics, Faculty of Pharmacy, University of Aix-Marseille, Marseille, France

    Joseph Ciccolini

Authors
  1. Alexandre Evrard
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  2. Joseph Ciccolini
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  3. Pierre Cuq
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  4. Jean-Paul Cano
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Editor information

Editors and Affiliations

  1. Cancer Research UK Centre for Cancer Therapeutics at the Institute of Cancer Research, Sutton, Surrey, UK

    Caroline J. Springer

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© 2004 Humana Press Inc.

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Evrard, A., Ciccolini, J., Cuq, P., Cano, JP. (2004). Enzyme-Prodrug Systems. In: Springer, C.J. (eds) Suicide Gene Therapy. Methods in Molecular Medicine™, vol 90. Humana Press. https://doi.org/10.1385/1-59259-429-8:263

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  • DOI: https://doi.org/10.1385/1-59259-429-8:263

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-971-1

  • Online ISBN: 978-1-59259-429-0

  • eBook Packages: Springer Protocols

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