Electroporation of E. coli
- Claire A. Woodall
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Electroporation, originally developed as a method to introduce DNA into eukaryotic cells (1), has subsequently been extensively used for bacterial transformation (2,3). This procedure is an effective method for the transfer of DNA to a wide range of Gram-negative bacteria, such as Escherichia coli, and reports indicate that 109 electro-transformants per microgram of DNA can be achieved in this species (4,5). Electroporation is probably the most efficient and reliable method for the transformation of E. coli strains using plasmid DNA.
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- Electroporation of E. coli
- Book Title
- E. coli Plasmid Vectors
- Book Subtitle
- Methods and Applications
- pp 55-59
- Print ISBN
- Online ISBN
- Series Title
- Methods in Molecular Biology™
- Series Volume
- Series ISSN
- Humana Press
- Copyright Holder
- Humana Press
- Additional Links
- Industry Sectors
- Editor Affiliations
- 1. University of California at Berkeley, School of Public Health
- 2. Department of Clinical Veterinary Medicine, University of Cambridge
- Author Affiliations
- 3. Department of Clinical Veterinary Medicine, University of Cambridge, Cambridge, UK
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