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PCR Detection of Microbial Pathogens pp 109–121Cite as

Isolation of Campylobacter and Identification byPCR

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Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 216))

Abstract

Campylobacter is now recognized worldwide as a leading cause of bacterial gastroenteritis in humans (1). Campylobacter species are common commensals in the intestinal tracts of poultry and livestock, and food products of animal origin are frequently associated with reported cases of illness (2). This chapter provides methods for the identification of C. jejuni and C. coli, which are the two species accounting for the majority of human infections. The protocols are routinely used in our laboratory and are intended to provide workers unfamiliar with Campylobacter culture and identification a useful set of methods to serve as a practical starting point.

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Author information

Authors and Affiliations

  1. Antimicrobial Resistance Research Unit, Richard B. Russell Agricultural Research Center, Agricultural Research Service, USDA, Athens, GA

    Mark D. Englen, Scott R. Ladely & Paula J. Fedorka-Cray

Authors
  1. Mark D. Englen
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  2. Scott R. Ladely
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  3. Paula J. Fedorka-Cray
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Editor information

Editors and Affiliations

  1. Federal Institute for Health Protection of Consumers and Veterinary Medicine (BgVV), Jena, Germany

    Konrad Sachse (Dr. Rer. Nat.) (Dr. Rer. Nat.)

  2. University of Bern, Bern, Switzerland

    Joachim Frey PhD

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© 2003 Humana Press Inc., Totowa, NJ

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D. Englen, M., R. Ladely, S., J. Fedorka-Cray, P. (2003). Isolation of Campylobacter and Identification byPCR. In: Sachse, K., Frey, J. (eds) PCR Detection of Microbial Pathogens. Methods in Molecular Biology™, vol 216. Humana Press. https://doi.org/10.1385/1-59259-344-5:109

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  • DOI: https://doi.org/10.1385/1-59259-344-5:109

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-049-6

  • Online ISBN: 978-1-59259-344-6

  • eBook Packages: Springer Protocols

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